Development of real-time PCR for the rapid detection of episomal Banana streak virus (BSV)

被引：21

作者：

Delanoy, M

Salmon, M

Kummert, J

Frison, E

Lepoivre, P ^{[1
]}

机构：

[1] Gembloux Agr Univ, Plant Pathol Unit, B-5030 Gembloux, Belgium

[2] Int Network Improvement Banana & Plantain INIBAP, Int Plant Genet Resources Inst IPGRI, F-34397 Montpellier, France

来源：

PLANT DISEASE | 2003年 / 87卷 / 01期

关键词：

badnavirus; long amplicon; minor groove binder (MGB); Musa; 5 ' nuclease assay;

D O I：

10.1094/PDIS.2003.87.1.33

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A real-time assay for the detection of episomal Banana streak virus (BSV; strain OL) in banana and plantains that carry integrated BSV sequences is described. Primers specific to the viral DNA were designed using the viral sequence integrated into the cv. Obino l'Ewai genome and the sequence of the genomic DNA of the infecting virus strain OL. They amplify a sequence of 1,336 bp that is detected in real-time by a short fluorogenic 3' minor groove binder DNA probe. This method enables reproducible and specific detection of episomal BSV from purified DNA as well as from crude extracts from infected plants. The assay is rapid, adaptable for large-scale experiments, and circumvents carryover problems.

引用

页码：33 / 38

页数：6

共 26 条

[1] Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays [J].