Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method

被引：5

作者：

Nakamura, Kosuke ^{[1
]}

Kondo, Kazunari ^{[1
]}

Akiyama, Hiroshi ^{[1
]}

Ishigaki, Takumi ^{[1
]}

Noguchi, Akio ^{[1
]}

Katsumata, Hiroshi ^{[2
]}

Takasaki, Kazuto ^{[2
]}

Futo, Satoshi ^{[2
]}

Sakata, Kozue ^{[1
]}

Fukuda, Nozomi ^{[1
]}

Mano, Junichi ^{[3
]}

Kitta, Kazumi ^{[3
]}

Tanaka, Hidenori ^{[4
]}

Akashi, Ryo ^{[4
]}

Nishimaki-Mogami, Tomoko ^{[1
]}

机构：

[1] Natl Inst Hlth Sci, Setagaya Ku, 1-18-1 Kamiyoga, Tokyo 1588501, Japan

[2] FASMAC CO LTD, 5-1-3 Midorigaoka, Atsugi, Kanagawa 2430041, Japan

[3] Natl Agr & Food Res Org, Natl Food Res Inst, Analyt Sci Div, 2-1-12 Kannondai, Tsukuba, Ibaraki 3058642, Japan

[4] Miyazaki Univ, Grad Sch Agr, 1-1 Gakuen Kibanadai Nishi, Miyazaki 8892192, Japan

来源：

FOOD CHEMISTRY | 2016年 / 205卷

关键词：

Genetically modified; Transgenic sequence; Next-generation sequencer; Genome sequence; Real-time PCR; Carica papaya L; PCR DETECTION METHODS; RINGSPOT VIRUS; MODIFIED ORGANISMS; SCREENING METHOD; RECOMBINANT-DNA; FOOD-PRODUCTS; T-DNA; RESISTANT; IDENTIFICATION; STRAINS;

D O I：

10.1016/j.foodchem.2016.02.157

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Identification of transgenic sequences in an unknown genetically modified (GM) papaya (Carica papaya L.) by whole genome sequence analysis was demonstrated. Whole genome sequence data were generated for a GM-positive fresh papaya fruit commodity detected in monitoring using real-time polymerase chain reaction (PCR). The sequences obtained were mapped against an open database for papaya genome sequence. Transgenic construct-and event-specific sequences were identified as a GM papaya developed to resist infection from a Papaya ringspot virus. Based on the transgenic sequences, a specific real-time PCR detection method for GM papaya applicable to various food commodities was developed. Whole genome sequence analysis enabled identifying unknown transgenic construct-and event-specific sequences in GM papaya and development of a reliable method for detecting them in papaya food commodities. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：272 / 279

页数：8

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