Evidence for a disease-promoting effect of Staphylococcus aureus-derived exotoxins in atopic dermatitis

被引:237
作者
Bunikowski, R
Mielke, MEA
Skarabis, H
Worm, M
Anagnostopoulos, I
Kolde, G
Wahn, U
Renz, H
机构
[1] Humboldt Univ, Dept Pediat Pneumol & Immunol, D-13353 Berlin, Germany
[2] Free Univ Berlin, Klinikum Benjamin Franklin, Inst Infect Dis, D-12200 Berlin, Germany
[3] Free Univ Berlin, Inst Sociol, Dept Stat, D-1000 Berlin, Germany
[4] Humboldt Univ, Charite, Dept Dermatol, Berlin, Germany
[5] Free Univ Berlin, Klinikum Benjamin Franklin, Inst Pathol, D-12200 Berlin, Germany
[6] Humboldt Univ, Inst Lab Med & Pathobiochem, Berlin, Germany
关键词
atopic dermatitis; Staphylococcus aureus; staphylococcus enterotoxins (SEA; SEB; SEC; SED; TSST-1);
D O I
10.1067/mai.2000.105528
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: The skin of patients with atopic dermatitis (AD) exhibits a striking susceptibility to colonization with Staphylococcus aureus. Some strains of S aureus secrete exotoxins with T-cell superantigen activity (toxigenic strains), and abnormal T-cell functions are known to play a critical role in AD. Objective: Our purpose was to examine the impact of superantigen production hy skin-colonizing S aureus on disease severity. Methods: In a cross-sectional study of 74 children with AD, the presence and density of toxigenic and nontoxigenic strains of S aureus was correlated with disease severity. In a subgroup of patients the T-cell receptor V beta repertoire of peripheral blood and lesional T cells was investigated and correlated with individual superantigen activity of skin-colonizing S aureus. Results: Fifty-three percent of children with AD were colonized with toxigenic strains of S aureus producing staphylococcal enterotoxin C, staphylococcal enterotoxin A, toxic shock syndrome toxin-1, staphylococcal enterotoxin B, and staphylococcal enterotoxin D in decreasing frequency. Children colonized with toxigenic S aureus strains had higher disease severity compared,vith the nontoxigenic and S aureus-negative groups. Patients colonized with toxigenic S aureus exhibited shifts in the intradermal T-cell receptor V beta repertoire that correspond to the respective superantigen-responsive T-cell subsets. Conclusion: The data demonstrate that S aureus-released exotoxins can modulate disease severity and dermal T-cell infiltration.
引用
收藏
页码:814 / 819
页数:6
相关论文
共 31 条
[1]  
ALOUF JE, 1991, SOURCEBOOK BACTERIAL, P367
[2]   Role of the T cell receptor α chain in stabilizing TCR-superantigen-MHC class II complexes [J].
Andersen, PS ;
Lavoie, PM ;
Sékaly, RP ;
Churchill, H ;
Kranz, DM ;
Schlievert, PM ;
Karjalainen, K ;
Mariuzza, RA .
IMMUNITY, 1999, 10 (04) :473-483
[3]   ALPHA-TOXIN OF STAPHYLOCOCCUS-AUREUS [J].
BHAKDI, S ;
TRANUMJENSEN, J .
MICROBIOLOGICAL REVIEWS, 1991, 55 (04) :733-751
[4]   IMMUNOENZYMATIC LABELING OF MONOCLONAL-ANTIBODIES USING IMMUNE-COMPLEXES OF ALKALINE-PHOSPHATASE AND MONOCLONAL ANTI-ALKALINE PHOSPHATASE (APAAP COMPLEXES) [J].
CORDELL, JL ;
FALINI, B ;
ERBER, WN ;
GHOSH, AK ;
ABDULAZIZ, Z ;
MACDONALD, S ;
PULFORD, KAF ;
STEIN, H ;
MASON, DY .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1984, 32 (02) :219-229
[5]   BACTERIAL-INFECTION AND ATOPIC ECZEMA [J].
DAVID, TJ ;
CAMBRIDGE, GC .
ARCHIVES OF DISEASE IN CHILDHOOD, 1986, 61 (01) :20-23
[6]   Nonspecific T-cell homing during inflammation in atopic dermatitis: Expression of cutaneous lymphocyte-associated antigen and integrin alpha E beta 7 on skin-infiltrating T cells [J].
deVries, IJM ;
LangeveldWildschut, EG ;
vanReijsen, FC ;
Bihari, IC ;
BruijnzeelKoomen, CAFM ;
Thepen, T .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1997, 100 (05) :694-701
[7]  
Ha SJ, 1998, ACTA DERM-VENEREOL, V78, P424
[8]   A Human-SCID mouse model for allergic immune responses:: Bacterial superantigen enhances skin inflammation and suppresses IgE production [J].
Herz, U ;
Schnoy, N ;
Borelli, S ;
Weigl, L ;
Käsbohrer, U ;
Daser, A ;
Wahn, U ;
Köttgen, E ;
Renz, H .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1998, 110 (03) :224-231
[9]   Role of T cells in atopic dermatitis - New aspects on the dynamics of cytokine production and the contribution of bacterial superantigens [J].
Herz, U ;
Bunikowski, R ;
Renz, H .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1998, 115 (03) :179-190
[10]   STAPHYLOCOCCAL SKIN COLONIZATION IN CHILDREN WITH ATOPIC-DERMATITIS - PREVALENCE, PERSISTENCE, AND TRANSMISSION OF TOXIGENIC AND NONTOXIGENIC STRAINS [J].
HOEGER, PH ;
LENZ, W ;
BOUTONNIER, A ;
FOURNIER, JM .
JOURNAL OF INFECTIOUS DISEASES, 1992, 165 (06) :1064-1068