Biosafety Test for Plant Growth-Promoting Bacteria: Proposed Environmental and Human Safety Index (EHSI) Protocol

被引:39
作者
Vilchez, Juan I. [1 ,2 ]
Navas, Alfonso [3 ]
Gonzalez-Lopez, Jesus [1 ,2 ]
Arcos, Susana C. [3 ]
Manzanera, Maximino [1 ,2 ]
机构
[1] Univ Granada, Inst Water Res, Granada, Spain
[2] Univ Granada, Dept Microbiol, Granada, Spain
[3] CSIC, Museo Nacl Ciencias Nat, Biodiversidad & Biol Evoiut, E-28006 Madrid, Spain
来源
FRONTIERS IN MICROBIOLOGY | 2016年 / 6卷
关键词
plant-growth-promoting-bacteria; inoculants; intentional-release; biosafety-index; ecotoxicity; CAENORHABDITIS-ELEGANS; PSEUDOMONAS-AERUGINOSA; ESCHERICHIA-COLI; GREENHOUSE CONDITIONS; VIBRIO-FISCHERI; RHIZOBACTERIA; REPRODUCTION; TOXICITY; EARTHWORMS; MICROFLORA;
D O I
10.3389/fmicb.2015.01514
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Plant growth promoting bacteria (PGPB) colonize plants and enhance their growth by different mechanisms. Some of these microorganisms may represent a potential threat to human, animal or plant health; however, their use might be approved in parts of Europe if they have been recommended as plant growth enhancers. The current regulatory framework has resulted in a fragmented, contradictory system, and there is an urgent need to establish harmonized protocols for the predictability, efficiency, consistency and especially the safety of PGPB for human and animal health and for the environment. In response to current efforts to update biosafety policies and provide alternative methods to replace the use of vertebrate animals, we propose a panel of tests and an evaluation system to reliably determine the biosafety of bacterial strains used as PGPB. Based on the results of different tests, we propose a scoring system to evaluate the safety of candidates for PGPB within the limitations of the assays used.
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页数:14
相关论文
共 48 条
[1]  
Adesemoye AO, 2008, CAN J MICROBIOL, V54, P876, DOI [10.1139/W08-081, 10.1139/w08-081]
[2]   Influence of inoculation with plant growth promoting rhizobacteria (PGPR) on tomato plant growth and nematode reproduction under greenhouse conditions [J].
Almaghrabi, Omar A. ;
Massoud, Samia I. ;
Abdelmoneim, Tamer S. .
SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 2013, 20 (01) :57-61
[3]   Laboratory toxicity studies demonstrate no adverse effects of Cry1Ab and Cry3Bb1 to larvae of Adalia bipunctata (Coleoptera: Coccinellidae): the importance of study design [J].
Alvarez-Alfageme, Fernando ;
Bigler, Franz ;
Romeis, Joerg .
TRANSGENIC RESEARCH, 2011, 20 (03) :467-479
[4]  
[Anonymous], 2002, The Delphi Method
[5]   An inducible activator produced by a Serratia proteamaculans strain and its soybean growth-promoting activity under greenhouse conditions [J].
Bai, YM ;
Souleimanov, A ;
Smith, DL .
JOURNAL OF EXPERIMENTAL BOTANY, 2002, 53 (373) :1495-1502
[6]  
BRENNER S, 1974, GENETICS, V77, P71
[7]   Earthworms influence metal transfer from soil to snails [J].
Coeurdassier, M. ;
Scheifler, R. ;
de Vaufleury, A. ;
Crini, N. ;
Saccomani, C. ;
Du Mont, L. Salomon ;
Badot, P. -M. .
APPLIED SOIL ECOLOGY, 2007, 35 (02) :302-310
[8]   Lethal paralysis of Caenorhabditis elegans by Pseudomonas aeruginosa [J].
Darby, C ;
Cosma, CL ;
Thomas, JH ;
Manoil, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (26) :15202-15207
[9]   Diet rapidly and reproducibly alters the human gut microbiome [J].
David, Lawrence A. ;
Maurice, Corinne F. ;
Carmody, Rachel N. ;
Gootenberg, David B. ;
Button, Julie E. ;
Wolfe, Benjamin E. ;
Ling, Alisha V. ;
Devlin, A. Sloan ;
Varma, Yug ;
Fischbach, Michael A. ;
Biddinger, Sudha B. ;
Dutton, Rachel J. ;
Turnbaugh, Peter J. .
NATURE, 2014, 505 (7484) :559-+
[10]   The sublethal effects of pesticides on beneficial arthropods [J].
Desneux, Nicolas ;
Decourtye, Axel ;
Delpuech, Jean-Marie .
ANNUAL REVIEW OF ENTOMOLOGY, 2007, 52 :81-106