Exosomes for mRNA delivery: a novel biotherapeutic strategy with hurdles and hope

被引：76

作者：

Aslan, Cynthia ^{[1
,2
]}

Kiaie, Seyed Hossein ^{[1
,3
]}

Zolbanin, Naime Majidi ^{[4
]}

Lotfinejad, Parisa ^{[1
,2
]}

Ramezani, Reihaneh ^{[5
]}

Kashanchi, Fatah ^{[6
]}

Jafari, Reza ^{[7
]}

机构：

[1] Tabriz Univ Med Sci, Immunol Res Ctr, Tabriz, Iran

[2] Tabriz Univ Med Sci, Fac Med, Dept Immunol, Tabriz, Iran

[3] Kermanshah Univ Med Sci, Nano Drug Delivery Res Ctr, Kermanshah, Iran

[4] Urmia Univ Med Sci, Sch Pharm, Dept Pharmacol & Toxicol, Orumiyeh, Iran

[5] Alzahra Univ, Women Res Ctr, Dept Biomed Sci, Tehran, Iran

[6] George Mason Univ, Sch Syst Biol, Lab Mol Virol, Manassas, VA USA

[7] Urmia Univ Med Sci, Solid Tumor Res Ctr, Cellular & Mol Med Res Inst, Shafa St,Ershad Blvd,POB 1138, Orumiyeh 57147, Iran

来源：

BMC BIOTECHNOLOGY | 2021年 / 21卷 / 01期

关键词：

mRNA therapy; Drug delivery; Exosomes; Extracellular vesicles; Immunogenicity; Toxicity; MILK-DERIVED EXOSOMES; SINGLE-STRANDED RNA; EXTRACELLULAR VESICLES; 5'-TRIPHOSPHATE RNA; PROTEIN EXPRESSION; GENE-TRANSFER; TUMOR; IMMUNOGENICITY; THERAPEUTICS; PSEUDOURIDINE;

D O I：

10.1186/s12896-021-00683-w

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Over the past decade, therapeutic messenger RNAs (mRNAs) have emerged as a highly promising new class of drugs for protein replacement therapies. Due to the recent developments, the incorporation of modified nucleotides in synthetic mRNAs can lead to maximizing protein expression and reducing adverse immunogenicity. Despite these stunning improvements, mRNA therapy is limited by the need for the development of safe and efficient carriers to protect the mRNA integrity for in vivo applications. Recently, leading candidates for in vivo drug delivery vehicles are cell-derived exosomes, which have fewer immunogenic responses. In the current study, the key hurdles facing mRNA-based therapeutics, with an emphasis on recent strategies to overcoming its immunogenicity and instability, were highlighted. Then the immunogenicity and toxicity of exosomes derived from various cell sources were mentioned in detail. Finally, an overview of the recent strategies in using exosomes for mRNA delivery in the treatment of multiple diseases was stated.

引用

页数：12

共 109 条

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