Protective effect of tanshinone IIA on human umbilical vein endothelial cell injured by hydrogen peroxide and its mechanism

被引：148
作者：
Lin, Rong [1 ]
Wang, Wei-Rong
Liu, Jun-Tian
Yang, Guang-De
Han, Chun-Jie
机构：
[1] Xi An Jiao Tong Univ, Sch Med, Dept Pharmacol, Xian, Shaanxi, Peoples R China
[2] Xi An Jiao Tong Univ, Minist Educ, Key Lab Environm & Genes Related Dis, Xian, Shaanxi, Peoples R China
[3] Xi An Jiao Tong Univ, Sch Med, Dept Pharm, Xian, Shaanxi, Peoples R China
来源：
JOURNAL OF ETHNOPHARMACOLOGY | 2006年 / 108卷 / 02期
基金：
中国国家自然科学基金;
关键词：
tanshinone IIA; hydrogen peroxide; apoptosis; nitric oxide; CD40;
D O I：
10.1016/j.jep.2006.05.004
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Tanshinone IIA (Tan HA) is isolated from Salvia miltiorrhiza, the root of which is widely used as a traditional Chinese medicine to treat atherosclerosis. The aim of the present study was to evaluate the putative protective effect of Tan IIA in a human umbilical vein endothelial cell line (ECV-304) injured by hydrogen peroxide in vitro and the mechanism of its protection. The percentage of cell viability was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The endothelial cell apoptosis and expression of cluster of differentiation 40 (CD40) were detected by flow cytometric analysis. Preincubation with Tan IIA significantly increased the viability of ECV-304 cell injured by hydrogen peroxide, which was accompanied with the increased nitric oxide level and superoxide dismutase activity in a dose-dependent manner. Moreover, cell apoptosis and CD40 expression were decreased in a dose-dependent manner. In conclusion, our data suggests that Tan IIA protects ECV-304 cell damage induced by hydrogen peroxide through its anti-oxidant effect and CD40 anti-inflammatory approach. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：217 / 222
页数：6
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