A Unique Family of Rigid Analogues of the GFP Chromophore with Tunable Two-Photon Action Cross-Sections for Biological Imaging

被引：109

作者：

Yuan, Lin ^{[1
]}

Lin, Weiying ^{[1
]}

Chen, Hua ^{[1
]}

Zhu, Sasa ^{[1
]}

He, Longwei ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 38期

关键词：

fluorescence imaging; fluorescent dyes; fluorescent probes; GFP chromophore analogues; two-photon dyes; GREEN FLUORESCENT PROTEIN; PROTON-TRANSFER; HYDROGEN-PEROXIDE; LIVING CELLS; PROBES; SENSORS; TISSUES; DESIGN; MODELS; THIOLS;

D O I：

10.1002/anie.201303179

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Conformationally restricted analogues of the GFP chromophore have been synthesized. The spectroscopic properties of GCTPOC, a unique family with tunable two-photon action cross-sections, were investigated. GCTPOC could be used as a robust two-photon platform for development of a two-photon fluorescent thiol sensor, which could stain endogenous thiols both in vitro and in vivo. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：10018 / 10022

页数：5

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