Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides

被引：23

作者：

Nishimura, Shinichi ^{[1
,2
]}

Ishii, Kumiko ^{[3
]}

Iwamoto, Kunihiko ^{[3
]}

Arita, Yuko ^{[1
]}

Matsunaga, Shigeki ^{[4
]}

Ohno-Iwashita, Yoshiko ^{[5
]}

Sato, Satoshi B. ^{[6
]}

Kakeya, Hideaki ^{[2
]}

Kobayashi, Toshihide ^{[3
]}

Yoshida, Minoru ^{[1
]}

机构：

[1] RIKEN Ctr Sustainable Resource Sci, Chem Genom Res Grp, Saitama, Japan

[2] Kyoto Univ, Div Bioinformat & Chem Genom, Grad Sch Pharmaceut Sci, Kyoto, Japan

[3] RIKEN, Lipid Biol Lab, Saitama, Japan

[4] Univ Tokyo, Lab Aquat Nat Prod Chem, Grad Sch Agr & Life Sci, Tokyo, Japan

[5] Iwaki Meisei Univ, Fac Pharm, Iwaki, Fukushima 970, Japan

[6] Kyoto Univ, Res Ctr Low Temp & Mat Sci, Kyoto, Japan

来源：

PLOS ONE | 2013年 / 8卷 / 12期

基金：

日本学术振兴会;

关键词：

CHOLESTEROL; TRANSPORT; GLYCOPEPTIDE; SWINHOEI; PEPTIDE; BINDING; PROTEIN; CELLS; RAFTS;

D O I：

10.1371/journal.pone.0083716

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cholesterol plays important roles in biological membranes. The cellular location where cholesterol molecules work is prerequisite information for understanding their dynamic action. Bioimaging probes for cholesterol molecules would be the most powerful means for unraveling the complex nature of lipid membranes. However, only a limited number of chemical or protein probes have been developed so far for cytological analysis. Here we show that fluorescently-labeled derivatives of theonellamides act as new sterol probes in mammalian cultured cells. The fluorescent probes recognized cholesterol molecules and bound to liposomes in a cholesterol-concentration dependent manner. The probes showed patchy distribution in the plasma membrane, while they stained specific organelle in the cytoplasm. These data suggest that fTNMs will be valuable sterol probes for studies on the role of sterols in the biological membrane under a variety of experimental conditions.

引用

页数：6

共 28 条

[21] THE USE AND ABUSE OF FILIPIN TO LOCALIZE CHOLESTEROL IN MEMBRANES [J].

MILLER, RG .

CELL BIOLOGY INTERNATIONAL REPORTS, 1984, 8 (07) :519-535

[22] Characterization of a cis-Golgi matrix protein, GM130 [J].

Nakamura, N ;

Rabouille, C ;

Watson, R ;

Nilsson, T ;

Hui, N ;

Slusarewicz, P ;

Kreis, TE ;

Warren, G .

JOURNAL OF CELL BIOLOGY, 1995, 131 (06) :1715-1726

[23]

Nishimura S, 2010, NAT CHEM BIOL, V6, P519, DOI [10.1038/NCHEMBIO.387, 10.1038/nchembio.387]

[24]

Ohno-Iwashita Y, 2010, SUBCELL BIOCHEM, V51, P597, DOI 10.1007/978-90-481-8622-8_22

[25] Distribution and transport of cholesterol-rich membrane domains monitored by a membrane-impermeant fluorescent polyethylene glycol-derivatized cholesterol [J].

Sato, SB ;

Ishii, K ;

Makino, A ;

Iwabuchi, K ;

Yamaji-Hasegawa, A ;

Senoh, Y ;

Nagaoka, I ;

Sakuraba, H ;

Kobayashi, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (22) :23790-23796

[26] Theopalauamide, a bicyclic glycopeptide from filamentous bacterial symbionts of the lithistid sponge Theonella swinhoei from Palau and Mozambique [J].

Schmidt, EW ;

Bewley, CA ;

Faulkner, DJ .

JOURNAL OF ORGANIC CHEMISTRY, 1998, 63 (04) :1254-1258

[27] Model systems, lipid rafts, and cell membranes [J].

Simons, K ;

Vaz, WLC .

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 2004, 33 :269-295

[28] Selective binding of perfringolysin O derivative to cholesterol-rich membrane microdomains (rafts) [J].

Waheed, AA ;

Shimada, Y ;

Heijnen, HFG ;

Nakamura, M ;

Inomata, M ;

Hayashi, M ;

Iwashita, S ;

Slot, JW ;

Ohno-Iwashita, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (09) :4926-4931

← 1 2 3 →