Somatic cell nuclear transfer: failures, successes and the challenges ahead

被引：49
作者：
Czernik, Marta [1 ,2 ]
Anzalone, Debora A. [1 ]
Palazzese, Luca [1 ]
Oikawa, Mami [3 ,4 ]
Loi, Pasqualino [1 ]
机构：
[1] Univ Teramo, Fac Vet Med, Teramo, Italy
[2] Polish Acad Sci, Dept Expt Embryol, Inst Genet & Anim Breeding, Jastrzebiec, Poland
[3] Natl Inst Nat Sci, Ctr Genet Anal Behav, Natl Inst Physiol Sci, Okazaki, Aichi, Japan
[4] Univ Cambridge, Wellcome Trust, Canc Res UK Gurdon Inst, Cambridge CB2 1QN, England
来源：
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY | 2019年 / 63卷 / 3-5期
基金：
欧盟地平线“2020”;
关键词：
somatic cell nuclear transfer; SCNT; nuclear reprogramming; mitochondria; RNAI-MEDIATED KNOCKDOWN; TRICHOSTATIN-A TREATMENT; FULL-TERM DEVELOPMENT; IN-VITRO DEVELOPMENT; MOUSE CLONING; MITOCHONDRIAL FUSION; HISTONE ACETYLATION; CLONED MICE; EXOGENOUS EXPRESSION; HANDMADE CLONING;
D O I：
10.1387/ijdb.180324mc
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Somatic cell nuclear transfer (SCNT) has a broad spectrum of potential applications, including rescue of endangered species, production of transgenic animals, drug production, and regenerative medicine. Unfortunately, the efficiency of SCNT is still disappointingly low. Many factors affecting cloning procedures have been described in several previous reviews; here we review the most effective improvements in SCNT, with a special emphasis on the effect of mitochondrial defects on SCNT embryo/foetus development, an issue never touched upon before.
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收藏
页码：123 / 130
页数：8
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