3D Nanostructured Multielectrode Arrays: Fabrication, Electrochemical Characterization, and Evaluation of Cell-Electrode Adhesion

被引:19
作者
Decker, Dominique [1 ]
Hempelmann, Rolf [2 ,3 ]
Natter, Harald [2 ,3 ]
Pirrung, Melissa [1 ]
Rabe, Holger [1 ]
Schaefer, Karl Herbert [1 ]
Saumer, Monika [1 ]
机构
[1] Univ Appl Sci Kaiserslautern, Dept Microsyst Technol, Amer Str 1, D-66482 Zweibrucken, Germany
[2] Saarland Univ, Transferctr Sustainable Electrochem, Campus Dudweiler,Markt Zeile 3, D-66125 Saarbrucken, Germany
[3] KIST Europe, Campus Dudweiler,Markt Zeile 3, D-66125 Saarbrucken, Germany
来源
ADVANCED MATERIALS TECHNOLOGIES | 2019年 / 4卷 / 02期
关键词
3D nanostructures; cell-electrode coupling; impedance; multielectrode arrays; nanoimprint lithography; MICROELECTRODE ARRAY; MAMMALIAN NEURONS; ACTION-POTENTIALS; SYSTEM; RECORDINGS; DESIGN; MEA;
D O I
10.1002/admt.201800436
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
3D nanostructures on top of planar multielectrode array (MEA) electrodes increase the surface area and can offer a tight and stable cell-electrode interface, thus leading to a crucial increase of the signal-to-noise ratio during measurement. However, each individual cell type might need specific dimensions and an arrangement of nanostructures that fits ideally to a specific cell type. Therefore, a fabrication method of 3D nanostructured MEA chips based on nanoimprint lithography, gold electroplating, and microstructuring techniques is presented which allows the fabrication of a whole set of MEA chips with different nanostructure layouts in one single approach. The chips are characterized using electrochemical methods, atomic force, and scanning electron microscopy. Furthermore, an impedance measurement method is presented to quantify cell-electrode adhesion of nanostructured and unstructured electrodes using the human embryonic kidney cell line (HEK 293). Double-layer capacitance, transferred charge, and impedance values of different nanostructure layouts revealed a significant improvement compared to unstructured electrodes. The improvement strongly correlates with the increase of the electrochemical active surface area. Impedance measurement with impedance-stable HEK 293 cells allows discriminating differences of cell adhesion between the individual nanostructure layouts. A significant increase or decrease of cell-electrode coupling depending on the nanostructure layout is demonstrated.
引用
收藏
页数:10
相关论文
共 51 条
  • [1] Conducting-polymer nanotubes for controlled drug release
    Abidian, MR
    Kim, DH
    Martin, DC
    [J]. ADVANCED MATERIALS, 2006, 18 (04) : 405 - +
  • [2] [Anonymous], 1908, BIOMETRIKA, V6, P1
  • [3] Antensteiner M., 2017, 39 ANN INT C IEEE EN, P1881
  • [4] Conducting Polymer Microcups for Organic Bioelectronics and Drug Delivery Applications
    Antensteiner, Martin
    Khorrami, Milad
    Fallahianbijan, Fatemeh
    Borhan, Ali
    Abidian, Mohammad Reza
    [J]. ADVANCED MATERIALS, 2017, 29 (39)
  • [5] Nanostructured gold microelectrodes for extracellular recording from electrogenic cells
    Brueggemann, D.
    Wolfrum, B.
    Maybeck, V.
    Mourzina, Y.
    Jansen, M.
    Offenhaeusser, A.
    [J]. NANOTECHNOLOGY, 2011, 22 (26)
  • [6] Dambrowsky N., 2007, THESIS
  • [7] Cells Adhering to 3D Vertical Nanostructures: Cell Membrane Reshaping without Stable Internalization
    Dipalo, Michele
    McGuire, Allister F.
    Lou, Hsin-Ya
    Caprettini, Valeria
    Melle, Giovanni
    Bruno, Giulia
    Lubrano, Claudia
    Matino, Laura
    Li, Xiao
    De Angelis, Francesco
    Cui, Bianxiao
    Santoro, Francesca
    [J]. NANO LETTERS, 2018, 18 (09) : 6100 - 6105
  • [8] Intracellular and Extracellular Recording of Spontaneous Action Potentials in Mammalian Neurons and Cardiac Cells with 3D Plasmonic Nanoelectrodes
    Dipalo, Michele
    Amin, Hayder
    Lovato, Laura
    Moia, Fabio
    Caprettini, Valeria
    Messina, Gabriele C.
    Tantussi, Francesco
    Berdondini, Luca
    De Angelis, Francesco
    [J]. NANO LETTERS, 2017, 17 (06) : 3932 - 3939
  • [9] 3D plasmonic nanoantennas integrated with MEA biosensors
    Dipalo, Michele
    Messina, Gabriele C.
    Amin, Hayder
    La Rocca, Rosanna
    Shalabaeva, Victoria
    Simi, Alessandro
    Maccione, Alessandro
    Zilio, Pierfrancesco
    Berdondini, Luca
    De Angelis, Francesco
    [J]. NANOSCALE, 2015, 7 (08) : 3703 - 3711
  • [10] Duan XJ, 2012, NAT NANOTECHNOL, V7, P174, DOI [10.1038/NNANO.2011.223, 10.1038/nnano.2011.223]