Ischemic preconditioning and the gene expression of enteric endothelial cell biology of rats submitted to intestinal ischemia and reperfusion

被引：5

作者：

Taha, Murched Omar ^{[1
]}

Ferreira, Regiane Miranda ^{[2
]}

Abrahao Taha, Nabiha Saadi ^{[3
]}

Monteiro, Hugo Pequeno ^{[4
]}

Caricati-Neto, Afonso ^{[2
]}

Oliveira-Junior, Itamar Souza ^{[5
]}

Fagundes, Djalma Jose ^{[1
]}

机构：

[1] Univ Fed Sao Paulo, Dept Surg, Operat Tech & Expt Surg Div, Sao Paulo, Brazil

[2] Univ Fed Sao Paulo, Dept Pharmacol, Sao Paulo, Brazil

[3] Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil

[4] Univ Fed Sao Paulo, Dept Biochem, Sao Paulo, Brazil

[5] Univ Fed Sao Paulo, Div Anesthesiol Pain & Intens Therapy, Sao Paulo, Brazil

来源：

ACTA CIRURGICA BRASILEIRA | 2013年 / 28卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

Ischemia; Reperfusion Injury; Endothelial Cells; Gene Expression Profiling; Rats; APOPTOSIS; TRANSPLANTATION; INHIBITOR; INJURY; PROTEINS; THERAPY;

D O I：

10.1590/S0102-86502013000300002

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

PURPOSE: To investigate the effects of ischemic preconditioning (IPC) on the expression of pro and anti-apoptotic genes in rat endothelial cells undergoing enteric ischemia (I) and reperfusion (R). METHODS: Thirty rats underwent clamping of the superior mesenteric vessels. Sham group (GS) laparotomy only; Ischemia (GI): intestinal ischemia (60 min); Ischemia and Reperfusion (GIR): ischemia (60 min) and reperfusion (120 min); Ischemia and intestinal ischemic preconditioning (GI + IPC) : 5 minutes of ischemia followed by 10 min of reperfusion before sustained ischemia (60 min) ischemia and reperfusion and IPC (GIR + IPC): 5 min ischemia followed by 10 min of reperfusion before sustained ischemia (60min) and reperfusion (120 min). Rat Endothelial Cell Biology (PCR array) to determine the expression of genes related to endothelial cell biology. RESULTS: Gene expression of pro-apoptotic markers (Casp1, Casp6, Cflar, Fas, and Pgl) was down regulated in GI+IPC and in GIR + IPC. In contrast, the expression of anti-apoptotic genes (Bcl2 and Naip2), was up-regulated in GI + IPC and in GIR + IPC. CONCLUSION: Ischemic preconditioning may protect against cell death caused by ischemia and reperfusion.

引用

页码：167 / 173

页数：7

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