A short peptide potentially promotes the healing of skin wound

被引：54
作者：
Song, Yongli [1 ]
Wu, Chunyun [1 ]
Zhang, Xinghe [2 ]
Bian, Wenxin [1 ]
Liu, Naixin [1 ]
Yin, Saige [1 ]
Yang, MeiFeng [1 ]
Luo, Mingying [1 ]
Tang, Jing [3 ]
Yang, Xinwang [1 ]
机构：
[1] Kunming Med Univ, Fac Basic Med Sci, Dept Anat & Histol & Embryol, Kunming 650500, Yunnan, Peoples R China
[2] Shandong Univ Tradit Chinese Med, Fac Acupuncture & Tuina, Jinan 250014, Shandong, Peoples R China
[3] Kunming Med Univ, Fac Basic Med Sci, Dept Biochem & Mol Biol, Kunming 650500, Yunnan, Peoples R China
来源：
BIOSCIENCE REPORTS | 2019年 / 39卷
关键词：
GROWTH-FACTOR RECEPTOR; ANTIMICROBIAL PEPTIDES; CELL-GROWTH; FACTOR-BETA; FROG; EXPRESSION; EPITHELIUM; DIVERSITY; MIGRATION; BIOLOGY;
D O I：
10.1042/BSR20181734
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Skin wound, a common form of skin damage in daily life, remains a serious challenge in clinical treatment. Bioactive peptides with high efficiency have been considered as potential therapeutic candidates for wound healing. In this report, a novel short linear peptide, with mature peptide sequence of 'GLLSGINAEWPC' and no obvious similarity with other known bioactive peptides, was identified by genomic method from the skin of odorous frog, Odorrana andersonii. Our results suggested that OA-GL12 (OA: abbreviation of species (O. andersonii), GL: two initial amino acids, 12: peptide length) obviously accelerated the scratch-healing of keratinocytes and human fibroblasts in a time-and concentration-dependent manner. Meanwhile, OA-GL12 showed significant effect in promoting the wound healing on the full-thickness skin wound model. Inflammatory assay results demonstrated that OA-GL12 induced the secretion of tumor necrosis factor (TNF) and transforming growth factor beta 1 (TGF-beta 1) on murine macrophage cell line (RAW264.7), which might explain the powerful accelerating capacity of wound healing. Moreover, results also indicated that epidermal growth factor receptor (EGFR) was involved in the mechanisms underlying the scratch-healing promoting activity of OA-GL12. In addition, OA-GL12 showed obvious free radical scavenging activity. Results supported that OA-GL12 did not exert risk in acute toxicity, hemolytic activity, and direct antibacterial activity. The remarkable effect of OA-GL12 on promoting wound healing verified in this research made it potential to be a novel template for the development of wound healing-promoting agents.
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页数：20
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[1]  
Badid C, 2000, HISTOL HISTOPATHOL, V15, P269, DOI 10.14670/HH-15.269
[2]   Growth factors and cytokines in wound healing [J].
Barrientos, Stephan ;
Stojadinovic, Olivera ;
Golinko, Michael S. ;
Brem, Harold ;
Tomic-Canic, Marjana .
WOUND REPAIR AND REGENERATION, 2008, 16 (05) :585-601
[3]   OA-GL21, a novel bioactive peptide from Odorrana andersonii, accelerated the healing of skin wounds [J].
Bian, Wenxin ;
Meng, Buliang ;
Li, Xiaojie ;
Wang, Siyuan ;
Cao, Xiaoqing ;
Liu, Naixin ;
Yang, Meifeng ;
Tang, Jing ;
Wang, Ying ;
Yang, Xinwang .
BIOSCIENCE REPORTS, 2018, 38
[4]   Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling [J].
Block, Ethan R. ;
Tolino, Michael A. ;
Lozano, Jennifer S. ;
Lathrop, Kira L. ;
Sullenberger, Rebecca S. ;
Mazie, Abigail R. ;
Klarlund, Jes K. .
MOLECULAR BIOLOGY OF THE CELL, 2010, 21 (13) :2172-2181
[5]   Frog skin epithelium: Electrolyte transport and chytridiomycosis [J].
Campbell, Craig R. ;
Voyles, Jamie ;
Cook, David I. ;
Dinudom, Anuwat .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2012, 44 (03) :431-434
[6]   Cathelicidin-OA1, a novel antioxidant peptide identified from an amphibian, accelerates skin wound healing (vol 8, 943, 2018) [J].
Cao, Xiaoqing ;
Wang, Ying ;
Wu, Chunyun ;
Li, Xiaojie ;
Fu, Zhe ;
Yang, Meifeng ;
Bian, Wenxin ;
Wang, Siyuan ;
Song, Yongli ;
Tang, Jing ;
Yang, Xinwang .
SCIENTIFIC REPORTS, 2018, 8
[7]   BLIMP1 transcriptionally induced by EGFR activation and post-translationally regulated by proteasome and lysosome is involved in keratinocyte differentiation, migration and inflammation [J].
Chang, Hua-Ching ;
Huang, Duen-Yi ;
Wu, Nan-Lin ;
Kannagi, Reiji ;
Wang, Li-Fang ;
Lin, Wan-Wan .
JOURNAL OF DERMATOLOGICAL SCIENCE, 2018, 92 (02) :151-161
[8]   Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis [J].
Chen, Zhongming ;
Yang, Xinwang ;
Liu, Zichao ;
Zeng, Lin ;
Lee, Wenhui ;
Zhang, Yun .
BIOCHIMIE, 2012, 94 (02) :328-334
[9]   Structural diversity and species distribution of host-defense peptides in frog skin secretions [J].
Conlon, J. Michael .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2011, 68 (13) :2303-2315
[10]   Ex vivo generation of a functional and regenerative wound epithelium from axolotl (Ambystoma mexicanum) skin [J].
Ferris, Donald R. ;
Satoh, Akira ;
Mandefro, Berhan ;
Cummings, Gillian M. ;
Gardiner, David M. ;
Rugg, Elizabeth L. .
DEVELOPMENT GROWTH & DIFFERENTIATION, 2010, 52 (08) :715-724
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