Enhanced binding of trigonal DNA-carbohydrate conjugates to lectin

被引：21

作者：

Matsui, Masayuki ^{[2
]}

Ebara, Yasuhito ^{[1
]}

机构：

[1] Kobe Univ, Grad Sch Human Dev & Environm, Nada Ku, Kobe, Hyogo 6578501, Japan

[2] Kobe Univ, Grad Sch Sci & Technol, Nada Ku, Kobe, Hyogo 6578501, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2012年 / 22卷 / 19期

关键词：

DNA-carbohydrate conjugate; Glycocluster; Multivalent interaction; Lectin; 3-Way junction DNA; CONCANAVALIN-A; JUNCTIONS; GLYCOCLUSTERS; CONSTRUCTION; RECOGNITION; STABILITY; PROTEINS; 3-WAY; ACID; SITE;

D O I：

10.1016/j.bmcl.2012.08.028

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Novel trigonal DNA-carbohydrate conjugates were prepared and evaluated to explore efficient carbohydrate-lectin interactions. Carbohydrate-modified oligonucleotides were enzymatically prepared, then hybridized to form 3-way junction DNAs. The thermal stabilities of the junctions were assessed by UV melting analysis and formation of constructs was confirmed by gel electrophoresis. Fluorescence titration assays revealed that the trigonal DNA-carbohydrate conjugates exhibit high affinity to lectins depending on the distribution of carbohydrates presented in each arm. These results suggest that self-assembled 3-way DNA architectures could offer a useful platform for controlling the spatial distribution of carbohydrates on conjugates and achieving more efficient molecular recognition. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：6139 / 6143

页数：5

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