Rainbow latex microspheres lateral flow immunoassay with smartphone-based device for simultaneous detection of three mycotoxins in cereals

被引：34

作者：

Chen, Jiayi ^{[1
]}

Luo, Pengjie ^{[2
]}

Liu, Zhiwei ^{[1
]}

He, Zixian ^{[1
]}

Pang, Yimeng ^{[1
]}

Lei, Hongtao ^{[1
]}

Xu, Zhenlin ^{[1
]}

Wang, Hong ^{[1
]}

Li, Xiangmei ^{[1
]}

机构：

[1] South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Peoples R China

[2] Chinese Acad Med Sci, China Natl Ctr Food Safety Risk Assessment, NHC Key Lab Food Safety Risk Assessment, Res Unit 2019RU014, Beijing 100022, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2022年 / 1221卷

关键词：

Latex microspheres; Lateral flow immunoassay; Aflatoxin B 1; Zearalenone; T-2; toxins; ASSAY; NANOPARTICLES; AFLATOXIN-B1;

D O I：

10.1016/j.aca.2022.340138

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The coexistent pollution of multiple mycotoxins displays a synergistic toxicity effect that significantly threatens human health. Therefore, it is essential to establish a rapid detection method for multi-mycotoxins in food. In this study, red, green, and blue latex microspheres (LMs) were applied as the aflatoxin B1 (AFB1), T-2 toxins (T-2), and zearalenone (ZEN) antibodies labeled tracer, respectively. Based on the principle of spatial resolution, a rainbow "traffic light" pattern latex microspheres lateral flow immunoassay (LMs-LFIA) integrated with a portable and user-friendly smartphone-based device was first developed to detect three kinds of mycotoxins simultaneously. The cut-off values of the method for AFB1/T-2/ZEN in cereals were 1/15/40 mu g kg-1, the limits of detection were 0.04/0.40/1.21 mu g kg-1, respectively. The recoveries ranged from 82.1% to 107.5%, with the coefficient of variation from 3.0% to 8.1%. A parallel analysis in 26 naturally contaminated cereal samples was confirmed by commercial ELISA kits; the results showed a good correlation (R2>0.99), indicating the practical reliability of the rainbow LMs-LFIA. This method provided a visually enjoyable, portable, and sensitive detection mode for multi-target detection of mycotoxins or other small molecule hazard factors in food.

引用

页数：9

共 42 条

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