Reduction of ferrylmyoglobin by beta-lactoglobulin

被引:55
作者
Ostdal, H
Daneshvar, B
Skibsted, LH
机构
[1] ROYAL VET & AGR UNIV, DEPT DAIRY & FOOD SCI, DK-1958 FREDERIKSBERG C, DENMARK
[2] NATL FOOD ACGY DENMARK, INST TOXICOL, DK-2860 SOBORG, DENMARK
关键词
ferrylmyoglobin; beta-lactoglobulin; cross-linking; bityrosine;
D O I
10.3109/10715769609088042
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Reduction of iron (IV) in ferrylmyoglobin in the presence of beta-lactoglobulin in aqueous solution is the result of two parallel reactions: (i) a so-called autoreduction, and (ii) reduction by beta-lactoglobulin in a second-order-reaction resulting in bityrosine formation in beta-lactoglobulin. In the pH-region investigated (5.4-7.4), the rate of reduction increased for both reactions with decreasing pH. The second order-reaction had for non-denatured beta-lactoglobulin the activation parameters: Delta H-double dagger = 45 kJ . mol(-1) and Delta S-double dagger = -93 J . mol(-1). K-1 at PH = 7.0 and ionic strength 0.16 (NaCl). Reduction of ferrylmyoglobin by beta-lactoglobulin denatured by heat (86 degrees C for 3 min) or by hydrostatic pressure (300 MPa for 15 min) resulted in formation of higher molecular weight species as detected by size-exclusion chromatography and by SDS-PAGE. No molecular weight changes were observed for reduction of ferrylmyoglobin by native beta-lactoglobulin. Detection of bityrosine in the native beta-lactoglobulin fraction after oxidation with ferrylmyoglobin indicated intra-molecular bityrosine formation. In heat-denatured beta-lactoglobulin bityrosine formation could be of intra-molecular and/or of inter-molecular origin, the latter being confirmed by size-exclusion chromatography.
引用
收藏
页码:429 / 438
页数:10
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