Separation of dansyl amino acid enantiomers by ligand-exchange capillary electrochromatography using an L-lysinamide-modified monolithic silica column

被引：5

作者：

Nishiyama, T ^{[1
]}

Chen, ZL ^{[1
]}

Nakagama, T ^{[1
]}

Uchiyama, K ^{[1
]}

Hobo, T ^{[1
]}

机构：

[1] Tokyo Metropolitan Univ, Grad Sch Engn, Dept Appl Chem, Hachioji, Tokyo 1920397, Japan

来源：

BUNSEKI KAGAKU | 2003年 / 52卷 / 11期

关键词：

chiral separation; capillary electrochromatography; monolithic column; dansyl amino acids; lysinamide;

D O I：

10.2116/bunsekikagaku.52.1005

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An L-lysinamide-modified monolithic column has been prepared by a sol-gel process and chemical modifications, and used for the enantioseparation of dansyl amino acids by both modes of capillary electrochromatography (CEC) and micro-high performance liquid chromatography (mu-HPLC). The electroosmotic flow (EOF), separation conditions and enantioselectivity have been investigated. The chiral selector of L-lysinamide loaded with Cu(II) ion generates a strong EOF, probably due to the nature of lysinamide containing two amino groups in a molecule. Besides, the separation behaviors in CEC and mu-HPLC have been compared.

引用

页码：1005 / 1009

页数：5

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