Examination of IGF2 and H19 loss of imprinting in bladder cancer

被引：114
作者：
Byun, Hyang-Min
Wong, Hui-Lee
Birnstein, Elliott Aaron
Wolff, Erika M.
Liang, Gangning
Yang, Allen S.
机构：
[1] Univ So Calif, Kenneth Norris Jr Comprehens Canc Ctr, Div Hematol, Los Angeles, CA 90033 USA
[2] Univ So Calif, Kenneth Norris Jr Comprehens Canc Ctr, Dept Biochem, Los Angeles, CA 90033 USA
[3] NCI, Viral Epidemiol Branch, Div Canc Epidemiol & Genet, NIH,Dept Hlth & Human Serv, Bethesda, MD 20892 USA
来源：
CANCER RESEARCH | 2007年 / 67卷 / 22期
关键词：
D O I：
10.1158/0008-5472.CAN-07-0329
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Loss of imprinting (1,01) is a common epigenetic event in cancer and may serve as an early biomarker in some cancers. To obtain a better understanding of 1,01, we studied 41 bladder tumors and their adjacent normal bladder mucosa. We found 2/9 (22.2%) cases that displayed LOI of IGF2 and 2/16 (12.5%) that had LOI of H19, as determined by the evaluation of mRNA for biallelic expression. In addition, we examined allele-specific methylation of the differentially methylated regions (DMR) of IGF2 and H19 using a new allele-specific pyrosequencing assay. We found that DNA methylation changes were a common finding (21/30, 70%) in the DMR regions, but could not clearly link DNA methylation changes with LOI as measured by biallelic expression. LOI and allele-specific DNA methylation changes are present in bladder cancer; however, a better understanding of the biology of LOI and its relationship to DNA methylation changes is needed before its use as an epigenetic biomarker.
引用
收藏
页码：10753 / 10758
页数：6
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