Liposomes with detachable polymer coating: Destabilization and fusion of dioleoylphosphatidylethanolamine vesicles triggered by cleavage of surface-grafted poly(ethylene glycol)

被引：166
作者：
Kirpotin, D
Hong, KL
Mullah, N
Papahadjopoulos, D
Zalipsky, S
机构：
[1] SEQUUS PHARMACEUT INC,MENLO PK,CA 94025
[2] UNIV CALIF SAN FRANCISCO,DEPT MOLEC & CELLULAR PHARMACOL,SAN FRANCISCO,CA 94143
[3] UNIV CALIF SAN FRANCISCO,CANC RES INST,SAN FRANCISCO,CA 94143
来源：
FEBS LETTERS | 1996年 / 388卷 / 2-3期
关键词：
liposomes; drug targeting; phosphatidylethanolamine; polyethylene glycol derivatives; membrane fusion; disulfide bond;
D O I：
10.1016/0014-5793(96)00521-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Plasma-stable liposomes (100 nn) were prepared from dioleoylphosphatidylethanolamine (DOPE) and 3-6 mol% of a new disulfide-linked poly(ethylene glycol)-phospholipid conjugate (mPEG-DTP-DSPE). In contrast to similar preparations containing non-cleavable PEG-phospholipid conjugate, thiolytic cleavage of the grafted polymer chains facilitated rapid and complete release of the liposome contents. Furthermore, the detachment of PEG from DOPE liposomes resulted in liposomal fusion, Finally, while formulation of pH-sensitive DOPE/cholesterol hemisuccinate liposomes with mPEG-DTP-DSPE abolished the pH sensitivity, cleavage of the PEG chains completely restored this property. These are the First examples of new useful properties of liposomes grafted with cleavable polymer.
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页码：115 / 118
页数：4
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