Deoxynivalenol fluorescence aptasensor based on AuCu bimetallic nanoclusters and MoS2

被引：14

作者：

Duan, Nuo ^{[1
,2
]}

Li, Changxin ^{[1
,2
]}

Song, Mingqian ^{[1
,2
]}

Ren, Kexin ^{[1
,2
]}

Wang, Zhouping ^{[1
,2
,3
]}

Wu, Shijia ^{[1
,2
,3
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

[2] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

[3] Jiangnan Univ, Int Joint Lab Food Safety, Wuxi 214122, Jiangsu, Peoples R China

来源：

MICROCHIMICA ACTA | 2022年 / 189卷 / 08期

关键词：

DON; Aptamer; AuCu bimetallic nanoclusters; MoS2; nanosheets; FRET; UP-CONVERSION NANOPARTICLES; RESONANCE ENERGY-TRANSFER; LATERAL FLOW IMMUNOASSAY; SENSITIVE DETECTION; WHEAT; LUMINESCENCE; ZEARALENONE; SELECTION; APTAMER;

D O I：

10.1007/s00604-022-05385-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Aptamers against deoxynivalenol (DON) were selected through capture-systematic evolution of ligands by exponential enrichment. Through isothermal titration calorimetry and fluorimetric assay, aptamer candidate DN-2 demonstrated good affinity to DON with K-d value of 40.36 +/- 6.32 nM. Accordingly, a Forster resonance energy transfer aptasensor was fabricated by using the aptamer DN-2 combined with AuCu bimetallic nanoclusters as energy donor and MoS2 nanosheets as energy acceptor. Under the optimal conditions, the fluorescence response was utilized for DON quantitative determination ranging from 5 to 100 ng/mL with a detection limit of 1.87 ng/mL. The practical application of this method was verified in maize flour samples and demonstrated a satisfied recovery of 94.6 similar to 103.1%. The obtained aptamers and their application in DON determination provide a new tool for DON monitoring in various foodstuff.

引用

页数：10

共 37 条

[1]

Belajová E, 2008, J FOOD NUTR RES-SLOV, V47, P189

[2] Dopamine-Modified AuCu Bimetallic Nanoclusters as Charge Transfer-Based Biosensors for Highly Sensitive Glycine Detection [J].

Chen, Zhichuan ;

Ding, Weihua ;

Gu, Yayun ;

Gao, Sheng ;

Yun, Damin ;

Wang, Chengniu ;

Li, Wenqing ;

Sun, Fei .

LANGMUIR, 2020, 36 (46) :13928-13936

[3] Deoxynivalenol and T-2 Toxin in Raw Feeds for Horses [J].

Cortinovis, Cristina ;

Battini, Monica ;

Caloni, Francesca .

JOURNAL OF EQUINE VETERINARY SCIENCE, 2012, 32 (02) :72-74

[4] An ssDNA library immobilized SELEX technique for selection of an aptamer against ractopamine [J].

Duan, Nuo ;

Gong, Wenhui ;

Wu, Shijia ;

Wang, Zhouping .

ANALYTICA CHIMICA ACTA, 2017, 961 :100-105

[5] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1990, 346 (6287) :818-822

[6] A novel aptameric nanobiosensor based on the self-assembled DNA-MoS2 nanosheet architecture for biomolecule detection [J].

Ge, Jia ;

Ou, En-Cai ;

Yu, Ru-Qin ;

Chu, Xia .

JOURNAL OF MATERIALS CHEMISTRY B, 2014, 2 (06) :625-628

[7] Natural occurrence of deoxynivalenol and zearalenone in wheat from Jiangsu province, China [J].

Ji, Fang ;

Xu, Jianhong ;

Liu, Xin ;

Yin, Xianchao ;

Shi, Jianrong .

FOOD CHEMISTRY, 2014, 157 :393-397

[8] Atomically Precise Colloidal Metal Nanoclusters and Nanoparticles: Fundamentals and Opportunities [J].

Jin, Rongchao ;

Zeng, Chenjie ;

Zhou, Meng ;

Chen, Yuxiang .

CHEMICAL REVIEWS, 2016, 116 (18) :10346-10413

[9]

Johnson W. C., 1996, Circular Dichroism and Conformational Analysis of Biomolecules, P433, DOI DOI 10.1007/978-1-4757-2508-7_12

[10] A novel aptamer-functionalized MoS2 nanosheet fluorescent biosensor for sensitive detection of prostate specific antigen [J].

Kong, Rong-Mei ;

Ding, Lu ;

Wang, Zhijie ;

You, Jinmao ;

Qu, Fengli .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2015, 407 (02) :369-377

← 1 2 3 4 →