Purification of embryonic stem cell-derived neurons by immunoisolation

被引:33
作者
Jüngling, K
Nägler, K
Pfrieger, FW
Gottmann, K
机构
[1] Ruhr Univ Bochum, Dept Cell Physiol, D-44780 Bochum, Germany
[2] Ctr Neurochim, Max Planck CNRS Grp, CNRS, UPR 2356, F-67084 Strasbourg, France
关键词
L1; antibodies; action potentials; glutamatergic synapses; GABAergic synapses; cell replacement;
D O I
10.1096/fj.03-0118fje
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The pluripotency and high proliferative capacity of embryonic stem (ES) cells (1-3) makes them an attractive source of different cell types for biomedical research and cell replacement therapies. A major prerequisite for these applications is the availability of a homogeneous population of the desired cell type. However, ES cell-derived material contains, for example, undifferentiated cells, which can cause tumor formation after transplantation into the brain (4). To avoid such unwanted side effects, effective purification of distinct types of cells needs to be developed. Here, we describe an immunoisolation procedure to purify neurons from in vitro differentiated mouse ES cells using an antibody against the neuronal cell adhesion molecule L1 (5, 6). Our procedure yields a pure population of differentiated neurons, which are electrically excitable and form excitatory, glutamatergic, and inhibitory GABAergic synapses. The ability to highly purify ES cell-derived neurons will boost their molecular characterization and the further exploration of their therapeutic potential.
引用
收藏
页码:2100 / +
页数:10
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