Transactivation of protein expression by rice HSP101 in planta and using Hsp101 as a selection marker for transformation

被引：26

作者：

Chang, Ching-Chun ^{[1
]}

Huang, Pin-Sheng ^{[1
]}

Lin, Huei-Ru ^{[1
]}

Lu, Chun-Hsien ^{[1
]}

机构：

[1] Natl Cheng Kung Univ, Inst Biotechnol, Tainan 701, Taiwan

来源：

PLANT AND CELL PHYSIOLOGY | 2007年 / 48卷 / 08期

关键词：

HSP101; selection marker; thermotolerance; transgenic tobacco; translational activator; MOLECULAR CHAPERONES; HEAT; ARABIDOPSIS; THERMOTOLERANCE; STRESS; GENE; TOLERANCE; MUTANTS; SYSTEM; YEAST;

D O I：

10.1093/pcp/pcm080

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plant HSP101 has dual activities, first, in conferring thermotolerance, and secondly, in serving as a translational activator. In this study, we introduced Oryza sativa Hsp101 (osHsp101) cDNA into tobacco by Agrohacterium-mediated transformation. Stable integration and expression of the transgene into the tobacco genome was demonstrated by Southern and Western blot analysis. Overexpression of osHSP101 had no noticeable effect on growth or development of the transgenic plants. Homozygous T-2 transgenic plants with overexpressed osHSP101 survived heat treatment better than untransformed control plants. In addition, taking advantage of conferring basal thermotolerance by plant HSP101, we were able to demonstrate the feasibility of using osHsp101 as a selection marker and select the transformants under high temperature in tobacco leaf disc transformation mediated by Agrobacterium. Furthermore, transgenic tobacco plants with overexpressed osHSP101 were able to enhance luciferase expression up to 2.9-fold more than untransformed plants in the progeny of reciprocally crossed with omega-luciferase reporter lines.

引用

页码：1098 / 1107

页数：10

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