Large scale in vivo risk assessment of bovine viral diarrhea virus (BVDV) transmission through transfer of bovine embryos produced via somatic cell nuclear transfer (SCNT)

被引：5
作者：
Gregg, K. [1 ]
Gosch, G. [2 ]
Guerra, T. [1 ]
Chen, S. H. [1 ]
Xiang, T. [1 ]
Broek, D. [2 ]
Bruner, B. [1 ]
Polejaeva, I. [1 ]
机构：
[1] Viagen Inc, Austin, TX 78727 USA
[2] Trans Ova Genet, Sioux Ctr, IA 51250 USA
来源：
THERIOGENOLOGY | 2010年 / 74卷 / 07期
关键词：
BVDV transmission; Risk Assessment; Somatic Cell Nuclear Transfer (SCNT); Embryo; Recipient; PERSISTENT INFECTION; VITRO; CALVES; PREVALENCE; CATTLE; DONOR; SEMEN; CALF; FERTILIZATION; ASSOCIATION;
D O I：
10.1016/j.theriogenology.2010.05.032
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The objective was to use the bovine viral diarrhea virus (BVDV) as a model to assess the risk of infectious disease transmission in the system of in vitro embryo production and transfer via somatic cell nuclear transfer (SCNT) technology. The risks of BVDV transmission in the SCNT embryo production were previously evaluated [1]. In that in vitro study, following standard operating procedures (SOP), including pre-nuclear transfer donor cell testing, oocyte decontamination and virus-free cell and embryo culture conditions, SCNT embryos produced were free of detectable viral RNA. The current study focused on the evaluation of the potential risk of disease transmission from SCNT embryos to recipients, and the risk of producing persistently infected animals via SCNT embryo transfer. Blood samples were collected from 553 recipients of SCNT embryos and 438 cloned calves and tested for the presence of BVDV viral RNA via a sensitive real time PCR method. All samples tested were negative. These results, in conjunction with the previous in vitro study, confirmed that the established SCNT embryo production and transfer system is safe and presents no detectable risk of disease transmission. (C) 2010 Elsevier Inc. All rights reserved.
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收藏
页码：1264 / 1270
页数：7
相关论文
共 28 条
[1]   CALVES BORN AFTER EMBRYO TRANSFER FROM DONORS PERSISTENTLY INFECTED WITH BVD VIRUS [J].
BAK, A ;
CALLESEN, H ;
MEYLING, A ;
GREVE, T .
VETERINARY RECORD, 1992, 131 (02) :37-37
[2]   Transmission of bovine viral diarrhea virus (BVDV) via in vitro-fertilized embryos to recipients, but not to their offspring [J].
Bielanski, A. ;
Algire, J. ;
Lalonde, A. ;
Nadin-Davis, S. .
THERIOGENOLOGY, 2009, 71 (03) :499-508
[3]   Disinfection procedures for controlling microorganisms in the semen and embryos of humans and farm animals [J].
Bielanski, A. .
THERIOGENOLOGY, 2007, 68 (01) :1-22
[4]   ISOLATION OF BOVINE HERPESVIRUS-1 (BHV-1) AND BOVINE VIRAL DIARRHEA VIRUS (BVDV) IN ASSOCIATION WITH THE IN-VITRO PRODUCTION OF BOVINE EMBRYOS [J].
BIELANSKI, A ;
LOEWEN, KS ;
DELCAMPO, MR ;
SIRARD, MA ;
WILLADSEN, S .
THERIOGENOLOGY, 1993, 40 (03) :531-538
[5]   Association of bovine embryos produced by in vitro fertilization with a noncytopathic strain of bovine viral diarrhea virus type II [J].
Bielanski, A ;
Sapp, T ;
Lutze-Wallace, C .
THERIOGENOLOGY, 1998, 49 (06) :1231-1238
[6]   Embryo transfer from donor cattle persistently infected with bovine viral diarrhea virus [J].
Brock, KV ;
Lapin, DR ;
Skrade, DR .
THERIOGENOLOGY, 1997, 47 (04) :837-844
[7]   Immunohistochemical evidence for the localization of bovine viral diarrhea virus, a single-stranded RNA virus, in ovarian oocytes in the cow [J].
Fray, MD ;
Prentice, H ;
Clarke, MC ;
Charleston, B .
VETERINARY PATHOLOGY, 1998, 35 (04) :253-259
[8]   Evaluation of diagnostic tests used for detection of bovine viral diarrhea virus and prevalence of subtypes 1a, 1b, and 2a in persistently infected cattle entering a feedlot [J].
Fulton, RW ;
Hessman, B ;
Johnson, BJ ;
Ridpath, JF ;
Saliki, JT ;
Burge, LJ ;
Sjeklocha, D ;
Confer, AW ;
Funk, RA .
JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION, 2006, 228 (04) :578-584
[9]   A single medium supports development of bovine embryos throughout maturation, fertilization and culture [J].
Gandhi, AP ;
Lane, M ;
Gardner, DK ;
Krisher, RL .
HUMAN REPRODUCTION, 2000, 15 (02) :395-401
[10]   Bovine viral diarrhea virus (BVDV): Epiderniologic concerns relative to semen and embryos [J].
Gard, J. A. ;
Givens, M. D. ;
Stringfellow, D. A. .
THERIOGENOLOGY, 2007, 68 (03) :434-442
123