Investigation of tissue inhibitor of metalloproteinases 1 in plasma from colorectal cancer patients and blood donors by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry

被引：0
作者：
Caspersen, M. B.
Sorensen, N. M.
Schrohl, A. S.
Iversen, P.
Nielsen, H. J.
Brunner, N.
机构：
[1] Univ Copenhagen, Dept Vet Pathobiol, Fac Life Sci, DK-1870 Frederiksberg C, Denmark
[2] Ciphergen Biosyst, Copenhagen, Denmark
[3] Hvidovre Univ Hosp, Dept Surg Gastroenterol, DK-2650 Hvidovre, Denmark
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS | 2007年 / 22卷 / 02期
关键词：
colorectal cancer; TIMP-1; SELDI TOF MS; ELISA; protein isoforms;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Early detection of colorectal cancer (CRC) improves patient survival. Plasma tissue inhibitor of metalloproteinases 1 (TIMP-1) measurements by enzyme-linked immunosorbent assay (ELISA) have been suggested as a new method for the early detection of CRC. To further investigate the nature of TIMP-1 in plasma, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI TOF MS) was used. TIMP-1 measurements of plasma from 16 healthy donors and 14 CRC patients were performed using TIMP-1 monoclonal antibody in SELDI TOF MS and ELISA. SELDI TOF MS applying an antibody to TIMP-1 revealed that human plasma TIMP-1 has a mass of 25.1 kDa and exhibits several isoforms. Both methods showed increased plasma TIMP-1 values for cancer patients as compared to healthy individuals. The p values for the separation of the groups were 0.0019 for ELISA and < 0.0001 for SELDI TOF MS. CRC did not fundamentally affect the appearance of TIMP-1 as evaluated by SELDI TOF MS.
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页码：89 / 94
页数：6
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