Protective response to subunit vaccination against intranasal Burkholderia mallei and B. pseudomallei challenge

被引：33

作者：

Whitlock, Gregory C. ^{[1
,4
]}

Deeraksa, Arpaporn ^{[2
]}

Qazi, Omar ^{[2
,5
]}

Judy, Barbara M. ^{[2
]}

Taylor, Katherine ^{[2
,6
]}

Propst, Katie L. ^{[8
]}

Duffy, Angie J. ^{[8
]}

Johnson, Kate ^{[5
]}

Kitto, G. Barrie ^{[5
,6
]}

Brown, Katherine A. ^{[5
,6
,7
]}

Dow, Steven W. ^{[8
]}

Torres, Alfredo G. ^{[1
,2
]}

Estes, D. Mark ^{[1
,2
,3
]}

机构：

[1] Univ Texas Med Branch, Dept Microbiol & Immunol, Galveston, TX 77555 USA

[2] Univ Texas Med Branch, Dept Pathol, Galveston, TX 77555 USA

[3] Univ Texas Med Branch, Sealy Ctr Vaccine Dev, Galveston, TX 77555 USA

[4] Univ Texas Med Branch, Dept Clin Lab Sci, Galveston, TX 77555 USA

[5] Univ Texas Austin, Inst Cellular & Mol Biol, Austin, TX 78712 USA

[6] Univ Texas Austin, Dept Chem & Biochem, Austin, TX 78712 USA

[7] Imperial Coll London, Dept Life Sci, London SW7 2AZ, England

[8] Colorado State Univ, Coll Vet Med, Dept Microbiol Immunol &Pathol, Ft Collins, CO 80523 USA

来源：

3RD VACCINE GLOBAL CONGRESS, SINGAPORE 2009 | 2010年 / 2卷 / 01期

关键词：

Burkholderia; B; mallei; pseudomallei; vaccine; subunit vaccination; intranasal infection; MELIOIDOSIS; INFECTION; POLYSACCHARIDE; IMMUNIZATION; PREDICTION; GLANDERS; MODEL; MICE;

D O I：

10.1016/j.provac.2010.03.013

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Burkholderia mallei and B. pseudomallei are Gram-negative pathogenic bacteria, responsible for the diseases glanders and melioidosis, respectively. Furthermore, there is currently no vaccine available against these Burkholderia species. In this study, we aimed to identify protective proteins against these pathogens. Immunization with recombinant B. mallei Hcp1 (type VI secreted/structural protein), BimA (autotransporter protein), BopA (type III secreted protein), and B. pseudomallei LolC (ABC transporter protein) generated significant protection against lethal inhaled B. mallei ATCC23344 and B. pseudomallei 1026b challenge. Immunization with BopA elicited the greatest protective activity, resulting in 100% and 60% survival against B. mallei and B. pseudomallei challenge, respectively. Moreover, sera from recovered mice demonstrated reactivity with the recombinant proteins. Dendritic cells stimulated with each of the different recombinant proteins showed distinct cytokine patterns. In addition, T cells from immunized mice produced IFN-following in vitro restimulation. These results indicated therefore that it was possible to elicit cross-protective immunity against both B. mallei and B. pseudomallei by vaccinating animals with one or more novel recombinant proteins identified in B. mallei. (C) 2010 Published by Elsevier Ltd.

引用

页码：73 / 77

页数：5

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