Luciferases with Tunable Emission Wavelengths

被引：63

作者：

Hiblot, Julien ^{[1
,2
]}

Yu, Qiuliyang ^{[1
,2
]}

Sabbadini, Marina D. B. ^{[1
]}

Reymond, Luc ^{[1
]}

Xue, Lin ^{[1
,2
]}

Schena, Alberto ^{[1
]}

Sallin, Olivier ^{[1
]}

Hill, Nicholas ^{[1
]}

Griss, Rudolf ^{[1
]}

Johnsson, Kai ^{[1
,2
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn, ISIC, NCCR Chem Biol, CH-1015 Lausanne, Switzerland

[2] Max Planck Inst Med Res, Dept Chem Biol, D-69120 Heidelberg, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2017年 / 56卷 / 46期

基金：

瑞士国家科学基金会;

关键词：

bioluminescent imaging; luciferase; self-labeling protein; sensors; therapeutic drug monitoring; SENSOR PROTEINS; IN-VIVO; CELLS;

D O I：

10.1002/anie.201708277

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP-tag or HaloTag7. Labeling of the self-labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.

引用

页码：14556 / 14560

页数：5

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