Evaluation of genotyping large numbers of Escherichia coli isolates by enterobacterial repetitive intergenic consensus-PCR

被引:60
作者
Meacham, KJ [1 ]
Zhang, LX [1 ]
Foxman, B [1 ]
Bauer, RJ [1 ]
Marrs, CF [1 ]
机构
[1] Univ Michigan, Sch Publ Hlth, Dept Epidemiol, Ann Arbor, MI 48109 USA
关键词
D O I
10.1128/JCM.41.11.5224-5226.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We analyzed a large collection of Escherichia coli isolates typed by enterobacterial repetitive intergenic consensus (ERIC)-PCR with BioNumerics gel analysis software. However, the interexperimental variation of ERIC-PCR caused the computer software to classify repeated isolates as different. ERIC-PCR should not be used as the sole determinant of genetic similarity when typing large numbers of bacterial isolates via computer software.
引用
收藏
页码:5224 / 5226
页数:3
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