Unconventional Therapy with IgY in a Psoriatic Mouse Model Targeting Gut Microbiome

被引:7
作者
Surcel, Mihaela [1 ]
Munteanu, Adriana [1 ,2 ]
Isvoranu, Gheorghita [3 ]
Ibram, Alef [4 ]
Caruntu, Constantin [5 ]
Constantin, Carolina [1 ,6 ]
Neagu, Monica [1 ,2 ,6 ]
机构
[1] Victor Babes Natl Inst Pathol, Immunol Dept, 99-101 Spl Independentei, Bucharest 050096, Romania
[2] Univ Bucharest, Fac Biol, Doctoral Sch Biol, 91-95 Spl Independentei, Bucharest 030018, Romania
[3] Victor Babes Natl Inst Pathol, Anim Husb, 99-101 Spl Independentei, Bucharest 050096, Romania
[4] Romvac Co SA, Res Lab, Voluntari 077190, Romania
[5] Carol Davila Univ Pharm & Med, Dept Physiol, 37 Dionisie Lupu St, Bucharest 020021, Romania
[6] Colentina Univ Hosp, Dept Pathol, Bucharest 020125, Romania
来源
JOURNAL OF PERSONALIZED MEDICINE | 2021年 / 11卷 / 09期
关键词
IgY; psoriatic dermatitis; imiquimod; inflammation; C57; BL/6; mice; NATURAL-KILLER-CELLS; EGG-YOLK ANTIBODIES; SKIN INFLAMMATION; IMMUNE; INHIBITION; VULGARIS; INDEX;
D O I
10.3390/jpm11090841
中图分类号
R19 [保健组织与事业(卫生事业管理)];
学科分类号
摘要
Psoriasis has a multifactorial pathogenesis and recently it was shown that alterations in the skin and intestinal microbiome are involved in the pathogenesis of psoriasis. Therefore, microbiome restoration becomes a promising preventive/therapy strategy in psoriasis. In our pre-clinical study design using a mice model of induced psoriatic dermatitis (Ps) we have tested the proof-of-concept that IgY raised against pathological human bacteria resistant to antibiotics can alleviate psoriatic lesions and restore deregulated immune cell parameters. Besides clinical evaluation of the mice and histology of the developed psoriatic lesions, cellular immune parameters were monitored. Immune cells populations/subpopulations from peripheral blood and spleen cell suspensions that follow the clinical improvement were assessed using flow cytometry. We have quantified T lymphocytes (CD3 epsilon(+)) with T-helper (CD4(+)CD8(-)) and T-suppressor/cytotoxic (CD8a(+)CD4(-)) subsets, B lymphocytes (CD3 epsilon(-)CD19(+)) and NK cells (CD3 epsilon(-)NK1.1(+)). Improved clinical evolution of the induced Ps along with the restoration of immune cells parameters were obtained when orally IgY was administered. We pin-point that IgY specific compound can be used as a possible pre-biotic-like alternative adjuvant in psoriasis.
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页数:21
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共 74 条
[1]   Natural Killer Cells: Development, Maturation, and Clinical Utilization [J].
Abel, Alex M. ;
Yang, Chao ;
Thakar, Monica S. ;
Malarkannan, Subramaniam .
FRONTIERS IN IMMUNOLOGY, 2018, 9
[2]   Evaluation of allergenicity of egg yolk immunoglobulin Y and other egg proteins by passive cutaneous anaphylaxis [J].
Akita, EM ;
Jang, CB ;
Kitts, DD ;
Nakai, S .
FOOD AND AGRICULTURAL IMMUNOLOGY, 1999, 11 (02) :191-201
[3]   CD28 T-cell costimulatory molecule expression in pemphigus vulgaris [J].
Alecu, M. ;
Ursaciuc, C. ;
Surcel, M. ;
Coman, G. ;
Ciotaru, D. ;
Dobre, M. .
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY, 2009, 23 (03) :288-291
[4]   NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells [J].
Alrefai, Hani ;
Muhammad, Khalid ;
Rudolf, Ronald ;
Duong Anh Thuy Pham ;
Klein-Hessling, Stefan ;
Patra, Amiya K. ;
Avots, Andris ;
Bukur, Valesca ;
Sahin, Ugur ;
Tenzer, Stefan ;
Goebeler, Matthias ;
Kerstan, Andreas ;
Serfling, Edgar .
NATURE COMMUNICATIONS, 2016, 7 :11724
[5]  
Alwawi Eihab A, 2008, Ther Clin Risk Manag, V4, P345
[6]   Inhibition of the Interleukin-36 Pathway for the Treatment of Generalized Pustular Psoriasis [J].
Bachelez, Herve ;
Choon, Siew-Eng ;
Marrakchi, Slaheddine ;
Burden, A. David ;
Tsai, Tsen-Fang ;
Morita, Akimichi ;
Turki, Hamida ;
Hall, David B. ;
Shear, Michael ;
Baum, Patrick ;
Padula, Steven J. ;
Thoma, Christian .
NEW ENGLAND JOURNAL OF MEDICINE, 2019, 380 (10) :981-983
[7]   Tildrakizumab efficacy and impact on quality of life up to 52 weeks in patients with moderate-to-severe psoriasis: a pooled analysis of two randomized controlled trials [J].
Blauvelt, A. ;
Sofen, H. ;
Papp, K. ;
Gooderham, M. ;
Tyring, S. ;
Zhao, Y. ;
Lowry, S. ;
Mendelsohn, A. ;
Parno, J. ;
Reich, K. .
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY, 2019, 33 (12) :2305-2312
[8]   Certolizumab pegol for the treatment of patients with moderate-to-severe chronic plaque psoriasis: pooled analysis of week 16 data from three randomized controlled trials [J].
Blauvelt, A. ;
Reich, K. ;
Lebwohl, M. ;
Burge, D. ;
Arendt, C. ;
Peterson, L. ;
Drew, J. ;
Rolleri, R. ;
Gottlieb, A. B. .
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY, 2019, 33 (03) :546-552
[9]   Models in the Research Process of Psoriasis [J].
Bochenska, Katarzyna ;
Smolinska, Elwira ;
Moskot, Marta ;
Jakobkiewicz-Banecka, Joanna ;
Gabig-Ciminska, Magdalena .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2017, 18 (12)
[10]   Skin and Gut Microbiome in Psoriasis: Gaining Insight Into the Pathophysiology of It and Finding Novel Therapeutic Strategies [J].
Chen, Lihui ;
Li, Jie ;
Zhu, Wu ;
Kuang, Yehong ;
Liu, Tao ;
Zhang, Wei ;
Chen, Xiang ;
Peng, Cong .
FRONTIERS IN MICROBIOLOGY, 2020, 11