Novel Pyropheophorbide Phosphatydic Acids Photosensitizer Combined EGFR siRNA Gene Therapy for Head and Neck Cancer Treatment

被引:9
作者
Yeh, Chia-Hsien [1 ]
Chen, Juan [2 ]
Zheng, Gang [2 ,3 ]
Huang, Leaf [4 ,5 ]
Hsu, Yih-Chih [1 ,5 ,6 ]
机构
[1] Chung Yuan Christian Univ, Dept Biosci Technol, Taoyuan 32023, Taiwan
[2] Univ Hlth Network UHN, Princess Margaret Canc Ctr, Toronto, ON M5G 1L7, Canada
[3] Univ Toronto, Dept Med Biophys, Toronto, ON M5S 1A1, Canada
[4] Univ N Carolina, Div Pharmacoengn & Mol Pharmaceut, Eshelman Sch Pharm, Chapel Hill, NC 27599 USA
[5] Chung Yuan Christian Univ, Ctr Canc Theranost & Commercializat Technol, Taoyuan 32023, Taiwan
[6] Chung Yuan Christian Univ, Ctr Nanotechnol, Taoyuan 32023, Taiwan
关键词
targeted delivery; head and neck cancer; nanoparticles; photodynamic therapy; GROWTH-FACTOR RECEPTOR; PHOTODYNAMIC THERAPY; FACTOR-ALPHA; DELIVERY; NANOPARTICLES;
D O I
10.3390/pharmaceutics13091435
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
This study combined two novel nanomedicines, a novel LCP Pyro PA photodynamic therapy (PDT) and LCP EGFR siRNA gene therapy, to treat head and neck cancer. A novel photosensitizer, pyropheophorbide phosphatydic acids (Pyro PA), was first modified into Lipid-Calcium phosphate nanoparticles named LCP Pyro PA NPs, and targeted with aminoethylanisamide as a novel PDT photosensitizer. EGFR siRNA was encapsulated into LCP NPs to silence EGFR expression. Measured sizes of LCP EGFR siRNA NPs and LCP Pyro-PA NPs were 34.9 +/- 3.0 and 20 nm respectively, and their zeta potentials were 51.8 +/- 1.8 and 52.0 +/- 7.6 mV respectively. In vitro studies showed that EGFR siRNA was effectively knocked down after photodynamic therapy (PDT) with significant inhibition of cancer growth. SCC4 or SAS xenografted nude mice were used to verify therapeutic efficacy. The LCP Control siRNA+PDT group of SCC4 and SAS showed significantly reduced tumor volume compared to the phosphate buffered saline (PBS) group. In the LCP-EGFR siRNA+LCP Pyro PA without light group and LCP EGFR siRNA + PBS with light group, SCC4 and SAS tumor volumes were reduced by similar to 140% and similar to 150%, respectively, compared to the PBS group. The LCP EGFR siRNA+PDT group of SCC4 and SAS tumor volumes were reduced by similar to 205% and similar to 220%, respectively, compared to the PBS group. Combined therapy showed significant tumor volume reduction compared to PBS, control siRNA, or PDT alone. QPCR results showed EGFR expression was significantly reduced after treatment with EGFR siRNA with PDT in SCC4 and SAS compared to control siRNA or PDT alone. Western blot results confirmed decreased EGFR protein expression in the combined therapy group. No toxic results were found in serum biomarkers. No inflammatory factors were found in heart, liver and kidney tissues. Results suggest that the novel LCP Pyro PA mediated PDT combined with LCP siEGFR NPs could be developed in clinical modalities for treating human head and neck cancer in the future.
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页数:17
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