Description of Bursaphelenchus microcarpae n. sp. isolated from Cryphalus abbreviatus emerged from Ficus microcarpa, with a report of B. carpini re-isolation

Two Bursaphelenchus spp. belonging to the eggersi group (subgroup 3) were isolated from bark beetles (Cryphalus spp.). One species. isolated from Cryphalus sp. cf . carpini that emerged from a log of an unidentified broad-leaved tree (considered to be Carpinus sp.) collected in Sugadaira. Nagano. Japan, was identified molecularly and typologically as B. carpini. The other species, isolated from C abbreviatus that emerged from a dead log of Ficus microcarpa collected in Okinawa, Japan, was differentiated molecularly from other eggersi group species. The new species is characterised by its three-lined lateral field, seven genital papillae with the posteriormost pair forming 'glandpapillae', short and stout male spicule with a strongly dorsally recurved and pointed condylus, small and rectangular male bursa! flap with a variable terminus. female vulva with a 'side flap'. broadly rounded female tail tip and molecular sequences of the ribosomal RNA region and partial mtCOI gene. The species is described and illustrated herein as B. microcarpae n. sp.