ROS-mediated time-varying cytotoxic effects on Phaeodactylum tricornutum under the stress of commercial naphthenic acids

被引：5

作者：

Lin, Zhihao ^{[1
]}

Zhang, Huanxin ^{[3
]}

Zhao, Xinyu ^{[1
,2
]}

Qu, Tongfei ^{[1
]}

Jun, Chen ^{[1
]}

Chen, Guan ^{[1
]}

Yi, Zhong ^{[1
]}

Hou, Chengzong ^{[1
]}

Tang, Xuexi ^{[1
,2
]}

Ying, Wang ^{[1
,2
]}

机构：

[1] Ocean Univ China, Coll Marine Life Sci, 5 Yushan Rd, Qingdao 266000, Peoples R China

[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China

[3] Shandong Normal Univ, Coll Geog & Environm, Jinan 250000, Peoples R China

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2022年 / 243卷

基金：

美国国家科学基金会;

关键词：

Naphthenic acids; Phaeodactylum tricornutum; Time-effect; Cellular cytotoxicity; Cell death types; PROCESS-AFFECTED WATERS; CELL-DEATH; OXIDATIVE STRESS; TOXICITY; APOPTOSIS; BIODEGRADATION; FRACTIONS; MIXTURES; GROWTH;

D O I：

10.1016/j.ecoenv.2022.114014

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The aquatic toxicity and ecological risks of naphthenic acids (NAs) in marine environments have attracted an increasing amount of attention. However, there remains a lack of methodologies for the long-term risk assess-ment of NAs on marine ecosystems after high acid crude oil spill accidents. In this study, using the model microalgae Phaeodactylum tricornutum as the target object, the time-effect manner under NAs stress is investi-gated for a continuous 24-144 h. We found that: 1) NAs caused photosynthetic damage and persistent oxidative stress that slowed the growth rate and limited the maximum growth of P. tricornutum population within 24 h to 144 h of exposure, especially under the high concentration treatment; 2) Within 144 h, NAs can cause oxidative stress to P. tricornutum. The damage to cell membrane and radical oxidative species (ROS) accumulation of P. tricornutum were observed as obvious time-effect; 3) Under NAs stress, the two types of cell death (accidental cell death and regulated cell death) of P. tricornutum cell mediated by ROS played different roles in the popu-lation growth inhibition of P. tricornutum. Moreover, regulated cell death of the P. tricornutum cell was accom-panied by PS externalization, DNA fragment and the G2/M phase stagnation acted as an adaptive regulatory mechanism under NAs stress. This explained the dose-time-effects of NAs on the population growth of P. tricornutum. Overall, the results suggested that NAs have a lasting effect on marine phytoplankton populations, and long-term risk assessments are required after high acid crude oil spill accidents. This is the first attempt to identify the different types of death at the cellular level to explain the time-effect toxicity at the population level of marine microalgae when exposed to NAs. This research will provide a new approach to facilitate further risk assessments for NAs and related contaminants in marine ecosystems.

引用

页数：11

共 53 条

[1] Chlorophyll hormesis: Are chlorophylls major components of stress biology in higher plants? [J].

Agathokleous, Evgenios ;

Feng, ZhaoZhong ;

Penuelas, Josep .

SCIENCE OF THE TOTAL ENVIRONMENT, 2020, 726

[2] Detection of naphthenic acid uptake into root and shoot tissues indicates a direct role for plants in the remediation of oil sands process-affected water [J].

Alberts, E. Mitchell ;

Wong, Jeremy ;

Hindle, Ralph ;

Degenhardt, Dani ;

Krygier, Richard ;

Turner, J. Raymond ;

Muench, G. Douglas .

SCIENCE OF THE TOTAL ENVIRONMENT, 2021, 795

[3]

[Anonymous], 1984, GUIDELINE TESTING CH

[4] Toxicity of naphthenic acids to invertebrates: Extracts from oil sands process-affected water versus commercial mixtures [J].

Bartlett, Adrienne J. ;

Frank, Richard A. ;

Gillis, Patricia L. ;

Parrott, Joanne L. ;

Marentette, Julie R. ;

Brown, Lisa R. ;

Hooey, Tina ;

Vanderveen, Ruth ;

McInnis, Rodney ;

Brunswick, Pamela ;

Shang, Dayue ;

Headley, John V. ;

Peru, Kerry M. ;

Hewitt, L. Mark .

ENVIRONMENTAL POLLUTION, 2017, 227 :271-279

[5] Iron starvation and culture age activate metacaspases and programmed cell death in the marine diatom Thalassiosira pseudonana [J].

Bidle, Kay D. ;

Bender, Sara J. .

EUKARYOTIC CELL, 2008, 7 (02) :223-236

[6] A review of the occurrence, analyses, toxicity, and biodegradation of naphthenic acids [J].

Clemente, JS ;

Fedorak, PM .

CHEMOSPHERE, 2005, 60 (05) :585-600

[7] Oxidative stress and regulated cell death in Parkinson's disease [J].

Dionisio, P. A. ;

Amaral, J. D. ;

Rodrigues, C. M. P. .

AGEING RESEARCH REVIEWS, 2021, 67

[8] Toxicity assessment of collected fractions from an extracted naphthenic acid mixture [J].

Frank, Richard A. ;

Kavanagh, Richard ;

Burnison, B. Kent ;

Arsenault, Gilles ;

Headley, John V. ;

Peru, Kerry M. ;

Van Der Kraak, Glen ;

Solomon, Keith R. .

CHEMOSPHERE, 2008, 72 (09) :1309-1314

[9] Essential versus accessory aspects of cell death: recommendations of the NCCD 2015 [J].

Galluzzi, L. ;

Bravo-San Pedro, J. M. ;

Vitale, I. ;

Aaronson, S. A. ;

Abrams, J. M. ;

Adam, D. ;

Alnemri, E. S. ;

Altucci, L. ;

Andrews, D. ;

Annicchiarico-Petruzzelli, M. ;

Baehrecke, E. H. ;

Bazan, N. G. ;

Bertrand, M. J. ;

Bianchi, K. ;

Blagosklonny, M. V. ;

Blomgren, K. ;

Borner, C. ;

Bredesen, D. E. ;

Brenner, C. ;

Campanella, M. ;

Candi, E. ;

Cecconi, F. ;

Chan, F. K. ;

Chandel, N. S. ;

Cheng, E. H. ;

Chipuk, J. E. ;

Cidlowski, J. A. ;

Ciechanover, A. ;

Dawson, T. M. ;

Dawson, V. L. ;

De laurenzi, V. ;

De Maria, R. ;

Debatin, K-M ;

Di Daniele, N. ;

Dixit, V. M. ;

Dynlacht, B. D. ;

El-Deiry, W. S. ;

Fimia, G. M. ;

Flavell, R. A. ;

Fulda, S. ;

Garrido, C. ;

Gougeon, M-L ;

Green, D. R. ;

Gronemeyer, H. ;

Hajnoczky, G. ;

Hardwick, J. M. ;

Hengartner, M. O. ;

Ichijo, H. ;

Joseph, B. ;

Jost, P. J. .

CELL DEATH AND DIFFERENTIATION, 2015, 22 (01) :58-73

[10]

Guillard R.R., 1975, Culture of marine invertebrate animals, P29, DOI [10.1007/978-1-4615-8714-9_3., DOI 10.1007/978-1-4615-8714-9_3, 10.1007/978-1-4615-8714-9_3]

← 1 2 3 4 5 6 →