Purification, crystallization and preliminary X-ray crystallographic analysis of nucleoside diphosphate kinase from rice

被引：4

作者：

Huang, JY

Chang, CY

Chang, T

Chen, CJ ^{[1
]}

机构：

[1] Natl Synchrotron Radiat Res Ctr, Xray Struct Biol Grp, Hsinchu 30077, Taiwan

[2] Natl Tsing Hua Univ, Dept Life Sci, Hsinchu 30077, Taiwan

[3] Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2003年 / 59卷

关键词：

D O I：

10.1107/S0907444903014616

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleoside diphosphate kinase (NDK) catalyses the transfer of the gamma-phosphoryl group from a nucleoside triphosphate (NTP) to a nucleoside diphosphate (NDP). NDK is involved in and required for coleoptile elongation in rice. The level of the enzyme changes during seed germination and the early stages of seedling growth. Recombinant rice NDK (rNDK) has been crystallized using the hanging-drop vapour-diffusion method. rNDK crystals diffracted to a resolution of 2.50 Angstrom and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.98, b = 182.26, c = 188.30 Angstrom. Preliminary analysis indicates there to be 12 rNDK molecules in each asymmetric unit, with a solvent content of 47.2%.

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页码：1648 / 1650

页数：3

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