Prevalence of toxocariasis in northeastern Brazil based on serology using recombinant Toxocara canis antigen

被引：33

作者：

Coelho, RDL

De Carvalho, LB

Perez, EP

Araki, K

Takeuchi, T

Ito, A

Aoki, T

Yamasaki, H ^{[1
]}

机构：

[1] Univ Fed Pernambuco, Lab Immunopatol Keizo Asami, Dept Bioquim, Recife, PE, Brazil

[2] Fiocruz MS, Dept Parasitol, Ctr Pesquisas Aggeu Magalhaes, Recife, PE, Brazil

[3] Tokyo Med & Dent Univ, Sect Environm Parasitol, Grad Sch, Tokyo, Japan

[4] Keio Univ, Sch Med, Dept Trop Med & Parasitol, Tokyo, Japan

[5] Juntendo Univ, Sch Med, Dept Mol & Cellular Parasitol, Tokyo, Japan

[6] Asahikawa Med Coll, Dept Parasitol, Asahikawa, Hokkaido, Japan

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2005年 / 72卷 / 01期

关键词：

D O I：

10.4269/ajtmh.2005.72.103

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

To evaluate the prevalence of toxocariasis in children in Jaboatao dos Guararapes, Pernambuco in northeastern Brazil, 215 serum samples were examined by an enzyme-linked immunosorbent assay (ELISA) using a recombinant Toxocara canis antigen. In the ELISA, 26 (12.1%) of 215 subjects were positive. In a dot-blot assay using 53 of 215 serum samples, the diagnostic results correlated with those obtained by the ELISA. Moreover, it has been confirmed that the recombinant T. canis antigen was highly specific for toxocariasis by ELISA using serum samples positive for antibody to Ascaris limbridicoides. Considering the specificity of the recombinant antigen to toxocariasis, the ELISA or dot-blot assay using the recombinant T. canis antigen is recommended in tropical and sub-tropical regions where various parasitic infections are commonly endemic.

引用

页码：103 / 107

页数：5

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