The Obesity Amelioration Effect in High-Fat-Diet Fed Mice of a Homogeneous Polysaccharide from Codonopsis pilosula

被引：11

作者：

Su, Qi ^{[1
,2
,3
]}

Huo, Jiangyan ^{[2
,3
]}

Wang, Yibin ^{[2
,3
]}

Zhou, Yang ^{[2
,3
]}

Luo, Dan ^{[2
,3
]}

Hou, Jinjun ^{[2
,3
]}

Zhang, Zijia ^{[2
,3
]}

Long, Huali ^{[2
,3
]}

Zhong, Xianchun ^{[2
,3
]}

Xie, Cen ^{[2
,3
]}

Lei, Min ^{[2
,3
]}

Liu, Yameng ^{[2
,3
]}

Wu, Wanying ^{[1
,2
,3
]}

机构：

[1] Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China

[2] Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Beijing 100049, Peoples R China

来源：

MOLECULES | 2022年 / 27卷 / 16期

关键词：

Codonopsis pilosula; polysaccharide; structural characterization; obesity; STRUCTURAL-CHARACTERIZATION; IN-VITRO;

D O I：

10.3390/molecules27165348

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A homogeneous polysaccharide coded as CPP-1 was extracted and purified from the root of Codonopsis pilosula (Franch.) Nannf. by water extraction, ethanol precipitation, and column chromatography. Its structure was analyzed by HPGPC-ELSD, HPLC, GC-MS, FT-IR, and NMR techniques. The results indicated that CPP-1 was composed of mannose (Man), glucose (Glc), galactose (Gal), and arabinose (Ara) at a molar ratio of 5.86 : 51.69 : 34.34 : 8.08. The methylation analysis revealed that the main glycosidic linkage types of CPP-1 were (1 ->)-linked-Glc residue, (1 -> 3)-linked-Glc residues, (1 -> 4)-linked-Gal residue, (1 -> 2,3,4)-linked-Glc residue, (1 ->)-linked-Man residue, (1 -> 3,4)-linked-Glc residue, and (1 ->)-linked-Ara residue. In vivo efficacy trial illustrated that CPP-1 supplements could alleviate HFD-induced mice obesity significantly, as well as improve obesity-induced disorders of glucose metabolism, alleviate insulin resistance, and improve the effects of lipid metabolism. The findings indicate that this polysaccharide has the potential for the treatment of obesity.

引用

页数：13

共 54 条

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