Structural and functional characterization of bovine G1P[5] rotavirus VP8*protein

被引：2

作者：

Dang, Lei ^{[1
,2
,3
]}

Su, Yunxi ^{[1
,2
]}

Qi, Jianxun ^{[4
]}

Wu, Zheng ^{[1
,2
]}

Li, Dandi ^{[1
,2
]}

Wang, Mengxuan ^{[1
,2
]}

Zhang, Qing ^{[1
,2
]}

Wang, Hong ^{[1
,2
]}

Bai, Ruixia ^{[5
]}

Duan, Zhaojun ^{[1
,2
]}

Sun, Xiaoman ^{[1
,2
]}

机构：

[1] Natl Hlth Commiss, Key Lab Med Virol & Viral Dis, Beijing 102206, Peoples R China

[2] China CDC, Natl Inst Viral Dis Control & Prevent, Beijing 102206, Peoples R China

[3] Inner Mongolia Hosp, Tradit Chinese Med, Hohhot 010059, Peoples R China

[4] Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China

[5] Inner Mongolia Med Univ, Hohhot 010059, Peoples R China

来源：

VIROLOGY | 2021年 / 563卷

基金：

中国国家自然科学基金;

关键词：

Bovine P[5] rotavirus; VP8*; X-ray crystallographic structure; Glycan binding specificity; Sialic acid; Histo-blood group antigen; SIALIC-ACID BINDING; STRAINS; RECOGNITION; SPECIFICITY; GANGLIOSIDE; VP8(STAR); DOMAIN; VP4;

D O I：

10.1016/j.virol.2021.08.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The widely used rotavirus (RV) vaccine, Rotateq, contained reassortment strains of human and bovine G1/2/3/ 4P[5] RVs. The functional and structural features of bovine G1P[5] VP8* were investigated. Bovine G1P[5] VP8* was identified to interact with sialic acids and sialic acid-containing glycans. In addition, P[5] VP8* recognized alpha-Gal histo-blood group antigens (HBGAs). Bovine G1P[5] VP8* did not hemagglutinate the tested red blood cells. The crystal structure of P[5] VP8* was determined at 1.7 angstrom. Structural superimposition revealed that P[5] VP8* was most close to human P[8] VP8*, while much further to VP8*s of porcine P[7] and rhesus P[3]. Sequence alignment showed that amino acids of the putative glycan binding site in P[5] VP8* were different to those in P[3]/P[7] VP8*s, indicating that P[5] VP8* may interact with glycans using different mechanism. This study provided more understanding of P[5] RV infection and the interactions of RV VP8* and glycans.

引用

页码：116 / 125

页数：10

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