Germ cell transplantation in infertility mouse

被引:0
作者
Xie XiaYang [1 ,2 ,3 ]
Teng Yong [4 ]
Ma LiangHong [5 ]
Liu LiJun [1 ]
Wang Xiang [6 ]
Song YingChing [2 ,3 ]
Xu Ping [1 ]
机构
[1] Chinese Acad Sci, Shanghai Lab Anim Ctr, Natl Rodent Lab Anim Resources, Shanghai Branch, Shanghai 201615, Peoples R China
[2] Med Coll Georgia, Inst Mol Med & Genet, Augusta, GA 30912 USA
[3] Xytex Res Inc, Augusta, GA 30912 USA
[4] Show How Biotech Inst, Beihai 536000, Peoples R China
[5] Hosp Affliated Ningxia Med Coll, Dept Urol, Yinchuan 750004, Peoples R China
[6] Fudan Univ, Huashan Hosp, Dept Urol, Shanghai 200040, Peoples R China
来源
CHINESE SCIENCE BULLETIN | 2008年 / 53卷 / 01期
基金
中国国家自然科学基金;
关键词
germ cell; transplantation; microinjection; infertility; GFP;
D O I
10.1007/s11434-008-0027-9
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
This work investigated the spermatogenesis in an infertility BALB/c-nu mouse model by reinfusing germline stem cells into seminiferous tubules. Donor germ cells were isolated from male FVB/NJ-GFP transgenic mice. Seminiferous tubule microinjection was applied to achieve intratubular germ cell transfer. The germ cells were injected into exposed testes of the infertility mice. We used green fluorescence and DNA analysis of donor cells from GFP transgenic mice as genetic marker. The natural mating and Southern blot methods were applied to analyze the effect of sperm cell transplantation and the sperm function after seminiferous tubule microinjection. The spermatogenesis was morphologically observed from the seminiferous tubules in 41/60 (68.33%) of the injected recipient mice using allogeneic donor cells. In the colonized testes, matured spermatozoa were seen in the lumen of the seminiferous tubules. In this research, BALB/c-nu infertility mouse model, the recipient animal, was used to avoid immunological rejection of donor cells, and germ cell transplantation was applied to overcome infertility caused by busulfan treatment. These results demonstrate that this technique of germ cell transplantation is of great use. Germ cell transplantation could be potentially valuable to oncological patients.
引用
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页码:70 / 75
页数:6
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