Resistance-Gene Cassettes Associated With Salmonella enterica Genotypes

被引：1

作者：

Bakhshi, Bita ^{[1
]}

Ghafari, Mohsen ^{[2
]}

Pourshafie, Mohammad R. ^{[3
]}

Zarbakhsh, Behnaz ^{[4
]}

Katouli, Mohammad ^{[5
]}

Rahbar, Mohammad ^{[6
]}

Hajia, Masoud ^{[6
]}

Hosseini-Aliabad, Neda ^{[2
]}

Boustanshenas, Mina ^{[7
]}

机构：

[1] Tarbiat Modares Univ, Fac Med Sci, Dept Bacteriol, Tehran, Iran

[2] Islamic Azad Univ, Sci & Res Branch, Dept Biol, Tehran, Iran

[3] Pasteur Inst Iran, Dept Bacteriol, Tehran, Iran

[4] Pasteur Inst Iran, Dept Mol Med, Tehran, Iran

[5] Univ Sunshine Coast, Dept Sci Hlth & Educ, Maroochydore, Qld, Australia

[6] Minist Hlth & Med Educ, Reference Hlth Labs Res Ctr, Dept Microbiol, Tehran, Iran

[7] Iran Univ Med Sci, Antimicrobial Resistance Res Ctr, Tehran, Iran

来源：

LABMEDICINE | 2015年 / 46卷 / 02期

关键词：

Salmonella enterica; serogrouping; integrons; genetic diversity; resistance; gene cassettes; FIELD GEL-ELECTROPHORESIS; CLASS; INTEGRONS; MULTIDRUG-RESISTANCE; ESCHERICHIA-COLI; UNITED-STATES; ANTIMICROBIAL RESISTANCE; ANTIBIOTIC-RESISTANCE; SEROVARS TYPHIMURIUM; VIRULENCE GENES; IDENTIFICATION;

D O I：

10.1309/LMFN8D17SOHQHGRP

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: The epidemiology of salmonellosis is complex because of the diversity and different serotypes of Salmonella enterica (S. enterica) that occur in different reservoirs and geographic incidences. Objectives: To determine the genotype distribution and resistance-gene content of 2 classes of integron among S. enterica isolates. Methods: Thirty-six S. enterica species were isolated and tested for their serological distribution and the resistance-gene contents of 2 classes of integron, as well as for their genetic diversity, using the pulsed-field gel electrophoresis (PFGE) genotyping method. Results: Serogroups E (36.1%) and D (30.5%) were dominant among the isolates. All of the isolates in serogroup D belonged to the serovar enteritidis. The aadA1 gene was found within all resistance-gene cassettes. We observed 4 common and 26 single pulsotypes among the isolates, which indicated a high degree of genetic diversity among the isolates. Using the PulseNet International standard protocol, it was found that these isolates were different from those reported previously in Iran. Conclusions: The presence of a few common and new pulsotypes among the isolates suggests the emergence and spread of new clones of S. enterica in Iran.

引用

页码：90 / 96

页数：7