The large N-terminal domain of Cdc25 protein of the yeast Saccharomyces cerevisiae is required for glucose-induced Ras2 activation

被引：11

作者：

Paiardi, Chiara ^{[1
]}

Belotti, Fiorella ^{[1
]}

Colombo, Sonia ^{[1
]}

Tisi, Rentata ^{[1
]}

Martegani, Enzo ^{[1
]}

机构：

[1] Univ Milano Bicocca, Dept Biotechnol & Biosci, I-20126 Milan, Italy

来源：

FEMS YEAST RESEARCH | 2007年 / 7卷 / 08期

关键词：

Cdc25; RasGTP; cAMP; Saccharomyces cerevisiae;

D O I：

10.1111/j.1567-1364.2007.00300.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Saccharomyces cerevisiae CDC25 gene encodes a guanine nucleotide exchange factor for Ras proteins whose catalytic domain is highly homologous to Ras-guanine nucleotide exchange factors from higher eukaryotes. In this study, glucose-induced Ras activation and cAMP response were investigated in mutants lacking the N-terminal domain of Cdc25 or where the entire CDC25 coding sequence was substituted by an expression cassette for a mammalian guanine nucleotide exchange factor catalytic domain. Our results suggest that an unregulated, low Ras guanine nucleotide exchange factor activity allows a normal glucose-induced cAMP signal that appears to be mediated mainly by the Gpr1/Gpa2 system, but it was not enough to sustain the glucose-induced increase of Ras2-GTP normally observed in a wild-type strain.

引用

页码：1270 / 1275

页数：6

共 24 条

[11] MACROMOLECULAR SYNTHESES IN THE CELL-CYCLE MUTANT CDC25 OF BUDDING YEAST [J].

MARTEGANI, E ;

VANONI, M ;

BARONI, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 144 (02) :205-210

[12] The SH3 domain of the S-cerevisiae Cdc25p binds adenylyl cyclase and facilitates Ras regulation of cAMP signalling [J].

Mintzer, KA ;

Field, J .

CELLULAR SIGNALLING, 1999, 11 (02) :127-135

[13] GLUCOSE-INDUCED CAMP SIGNALING IN SACCHAROMYCES-CEREVISIAE IS MEDIATED BY THE CDC25 PROTEIN [J].

MUNDER, T ;

KUNTZEL, H .

FEBS LETTERS, 1989, 242 (02) :341-345

[14] RIGOROUS FEEDBACK-CONTROL OF CAMP LEVELS IN SACCHAROMYCES-CEREVISIAE [J].

NIKAWA, J ;

CAMERON, S ;

TODA, T ;

FERGUSON, KM ;

WIGLER, M .

GENES & DEVELOPMENT, 1987, 1 (09) :931-937

[15] Glucose-induced cAMP signalling in yeast requires both a G-protein coupled receptor system for extracellular glucose detection and a separable hexose kinase-dependent sensing process [J].

Rolland, F ;

de Winde, JH ;

Lemaire, K ;

Boles, E ;

Thevelein, JM ;

Winderickx, J .

MOLECULAR MICROBIOLOGY, 2000, 38 (02) :348-358

[16] Role of guanine nucleotides in the regulation of the Ras/cAMP pathway in Saccharomyces cerevisiae [J].

Rudoni, S ;

Colombo, S ;

Coccetti, P ;

Martegani, E .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2001, 1538 (2-3) :181-189

[17] Association of yeast adenylyl cyclase with cyclase-associated protein CAP forms a second Ras-binding site which mediates its Ras-dependent activation [J].

Shima, F ;

Okada, T ;

Kido, M ;

Sen, H ;

Tanaka, Y ;

Tamada, M ;

Hu, CD ;

Yamawaki-Kataoka, Y ;

Kariya, KI ;

Kataoka, T .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (01) :26-33

[18] SACCHAROMYCES-CEREVISIAE GENES IRA1 AND IRA2 ENCODE PROTEINS THAT MAY BE FUNCTIONALLY EQUIVALENT TO MAMMALIAN RAS GTPASE ACTIVATING PROTEIN [J].

TANAKA, K ;

NAKAFUKU, M ;

SATOH, T ;

MARSHALL, MS ;

GIBBS, JB ;

MATSUMOTO, K ;

KAZIRO, Y ;

TOHE, A .

CELL, 1990, 60 (05) :803-807

[19] Novel sensing mechanisms and targets for the cAMP-protein kinase A pathway in the yeast Saccharomyces cerevisiae [J].

Thevelein, JM ;

de Winde, JH .

MOLECULAR MICROBIOLOGY, 1999, 33 (05) :904-918

[20] SIGNAL-TRANSDUCTION IN YEAST [J].

THEVELEIN, JM .

YEAST, 1994, 10 (13) :1753-1790

← 1 2 3 →