Determination of pKa values of organic bases in aqueous acetonitrile solutions using capillary electrophoresis

被引：45

作者：

Buckenmaier, SMC

McCalley, DV ^{[1
]}

Euerby, MR

机构：

[1] Univ W England, Ctr Res Analyt Methods & Sensors, Bristol BS16 1QY, Avon, England

[2] AstraZeneca R&D Charnwood Lund, Pharmaceut & Analyt R&D, Loughborough LE11 5RH, Leics, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 1004卷 / 1-2期

关键词：

ionisation constants; organic bases;

D O I：

10.1016/S0021-9673(03)00717-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary electrophoresis (CE) was used for the determination of ionisation constants (pK(a)) of a variety of organic bases in aqueous acetonitrile solutions over the range 0-60% (vW/v) acetonitrile. These bases are used as test compounds in HPLC column evaluation, thus knowledge of their pK(a) in hydro-organic solutions is useful. The base pK(a) decreased with acetonitrile concentration and significant shifts from the aqueous pK(a) (up to -0.8) were found using 60% acetonitrile. The CE application was confirmed to be very suitable for fast and accurate pK(a) measurement in aqueous organic solutions. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：71 / 79

页数：9

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