Metabolic engineering of Saccharomyces cerevisiae for the biotechnological production of succinic acid

被引：174

作者：

Raab, Andreas M. ^{[1
,2
]}

Gebhardt, Gabi ^{[3
]}

Bolotina, Natalia ^{[2
]}

Weuster-Botz, Dirk ^{[3
]}

Lang, Christine ^{[1
,2
]}

机构：

[1] Berlin Univ Technol, Inst Biotechnol, Dept Genet & Microbiol, D-13355 Berlin, Germany

[2] ORGANOBALANCE GmbH, D-13355 Berlin, Germany

[3] Tech Univ Munich, Inst Biochem Engn, D-85748 Garching, Germany

来源：

METABOLIC ENGINEERING | 2010年 / 12卷 / 06期

关键词：

Yeast; Saccharomyces cerevisiae; Succinic acid; Metabolic engineering; Production; MANNHEIMIA-SUCCINICIPRODUCENS LPK7; ESCHERICHIA-COLI; ISOCITRATE DEHYDROGENASE; GENE DISRUPTION; SAKE YEAST; CORYNEBACTERIUM-GLUTAMICUM; UBIQUINONE OXIDOREDUCTASE; ORGANIC-ACIDS; BOVINE RUMEN; EXPRESSION;

D O I：

10.1016/j.ymben.2010.08.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The production of bio-based succinic acid is receiving great attention, and several predominantly prokaryotic organisms have been evaluated for this purpose. In this study we report on the suitability of the highly acid- and osmotolerant yeast Saccharomyces cerevisiae as a succinic acid production host. We implemented a metabolic engineering strategy for the oxidative production of succinic acid in yeast by deletion of the genes SDH1, SDH2, IDH1 and IDP1. The engineered strains harbor a TCA cycle that is completely interrupted after the intermediates isocitrate and succinate. The strains show no serious growth constraints on glucose. In glucose-grown shake flask cultures, the quadruple deletion strain Delta sdh1 Delta sdh2 Delta idh1 Delta idp1 produces succinic acid at a titer of 3.62 gL(-1) (factor 4.8 compared to wild-type) at a yield of 0.11 mol (mol glucose)(-1). Succinic acid is not accumulated intracellularly. This makes the yeast S. cerevisiae a suitable and promising candidate for the biotechnological production of succinic acid on an industrial scale. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：518 / 525

页数：8

共 62 条

[61] Improved succinic acid production in the anaerobic culture of an Escherichia coli PflB ldhA double mutant as a result of enhanced anaplerotic activities in the preceding aerobic culture [J].

Wu, Hui ;

Li, Zhi-Min ;

Zhou, Li ;

Ye, Qin .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (24) :7837-7843

[62] IMPROVED M13 PHAGE CLONING VECTORS AND HOST STRAINS - NUCLEOTIDE-SEQUENCES OF THE M13MP18 AND PUC19 VECTORS [J].

YANISCHPERRON, C ;

VIEIRA, J ;

MESSING, J .

GENE, 1985, 33 (01) :103-119

← 1 2 3 4 5 6 7 →