Mechanistic investigation in ultrasound induced enhancement of enzymatic hydrolysis of invasive biomass species

被引：36

作者：

Borah, Arup Jyoti ^{[1
]}

Agarwal, Mayank ^{[2
]}

Poudyal, Manisha ^{[4
]}

Goyal, Arun ^{[1
,3
]}

Moholkar, Vijayanand S. ^{[1
,2
,4
]}

机构：

[1] Indian Inst Technol, Ctr Energy, Gauhati 781039, Assam, India

[2] Indian Inst Technol, Dept Chem Engn, Gauhati 781039, Assam, India

[3] Indian Inst Technol, Dept Biosci & Bioengn, Gauhati 781039, Assam, India

[4] Tezpur Univ, Dept Mol Biol & Biotechnol, Tezpur 784028, Assam, India

来源：

BIORESOURCE TECHNOLOGY | 2016年 / 213卷

关键词：

Enzymatic hydrolysis; Ultrasound; Cavitation; Conformational change; Genetic Algorithm; PROTEIN SECONDARY STRUCTURE; BIOETHANOL PRODUCTION; PARTHENIUM-HYSTEROPHORUS; CELLULOSE HYDROLYSIS; SACCHARUM-SPONTANEUM; WATER HYACINTH; PRETREATMENT; SACCHARIFICATION; FERMENTATION; OPTIMIZATION;

D O I：

10.1016/j.biortech.2016.02.024

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

This study has assessed four invasive weeds, viz. Saccharum spontaneum (SS), Mikania micrantha (MM), Lantana camara (LC) and Eichhornia crassipes (EC) for enzymatic hydrolysis prior to bioalcohol fermentation. Enzymatic hydrolysis of pretreated biomasses of weeds has been conducted with mechanical agitation and sonication under constant (non-optimum) conditions. Profiles of total reducible sugar release have been fitted to HCH-1 model of enzymatic hydrolysis using Genetic Algorithm. Trends in parameters of this model reveal physical mechanism of ultrasound-induced enhancement of enzymatic hydrolysis. Sonication accelerates hydrolysis kinetics by similar to 10-fold. This effect is contributed by several causes, attributed to intense micro-convection generated during sonication: (1) increase in reaction velocity, (2) increase in enzyme-substrate affinity, (3) reduction in product inhibition, and (4) enhancement of enzyme activity due to conformational changes in its secondary structure. Enhancement effect of sonication is revealed to be independent of conditions of enzymatic hydrolysis - whether optimum or nonoptimum. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：342 / 349

页数：8

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