Biotransformation of l-ornithine from l-arginine using whole-cell recombinant arginase

被引：11

作者：

Zhan, Yueping ^{[1
]}

Liu, Junzhong ^{[1
]}

Mao, Pingting ^{[1
]}

Zhang, Hongjuan ^{[1
]}

Liu, Qian ^{[1
]}

Jiao, Qingcai ^{[1
]}

机构：

[1] Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Sch Life Sci, Nanjing 210093, Jiangsu, Peoples R China

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2013年 / 29卷 / 11期

关键词：

Arginase; Whole-cell; Biotransformation; L-Arginine; L-Ornithine; INHIBITION; ADAPTATION;

D O I：

10.1007/s11274-013-1382-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A recombinant arginase was generated for a whole-cell biotransformation system to convert l-arginine to l-ornithine in Escherichia coli. The gene ARG1 coding arginase from Bos taurus liver was synthesized and expressed in E. coli BL21 (DE3) via pETDuet-1. The recombinant arginase was used to catalyze l-arginine to l-ornithine and urea. The reaction was optimal at pH 9.5 and 37 A degrees C. Manganese (10(-5) M) and Emulsifier OP-10 [0.033 % (v/v)] could promote arginase activity. In a scale up study, l-arginine conversion rate reached 98 % with a final concentration of 111.52 g l-ornithine/l.

引用

收藏

页码：2167 / 2172

页数：6

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