Oral toxicity study of certain plant extracts containing pyrrolizidine alkaloids

被引:0
作者
Seremet, Oana Cristina [1 ]
Barbuceanu, Florica [2 ]
Ionica, Floriana Elvira [3 ]
Margina, Denisa Marilena [4 ]
Gutu, Claudia Maria [5 ]
Olaru, Octavian Tudorel [6 ]
Ilie, Mihaela [5 ]
Gonciar, Veaceslav [7 ]
Negres, Simona [1 ]
Chirita, Cornel [1 ]
机构
[1] Carol Davila Univ Med & Pharm, Fac Pharm, Dept Pharmacol & Clin Pharm, Bucharest, Romania
[2] Inst Diag & Anim Hlth, Dept Morphopathol, Bucharest, Romania
[3] Univ Med & Pharm Craiova, Fac Pharm, 2 Petru Rares St, Craiova 200349, Romania
[4] Carol Davila Univ Med & Pharm, Fac Pharm, Dept Biochem, Bucharest, Romania
[5] Carol Davila Univ Med & Pharm, Fac Pharm, Dept Toxicol, Bucharest, Romania
[6] Carol Davila Univ Med & Pharm, Fac Pharm, Dept Pharmaceut Bot & Cell Biol, Bucharest, Romania
[7] Nicolae Testemitanu State Univ Med & Pharm, Fac Pharm, Dept Pharmacol & Clin Pharm, Kishinev, Moldova
关键词
pyrrolizidine alkaloids; hepatic toxicity; repeated dose toxicity; histopathological examination; ACTIVATION; INJURY;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Pyrrolizidine alkaloids (PAs) are a class of toxic compounds which are found in plants. Poisoning caused by these toxins is associated with acute and chronic liver damage. Tussilago tartars (coltsfoot), Petasites hybridus (common butterbur), Senecio vemalis (eastem groundsel) and Symphytum officinale (comfrey) are traditional phytotherapic species, which beside the therapeutic bioactive compounds contain PAs. The aim of the paper was to assess the safety of some dry extracts obtained from these species. For the determination of acute toxicity, Organization for Economic Cooperation and Development (OECD) Guideline No. 423 was used. For the determination of repeated dose oral toxicity, Senecionis vemalis herba and Symphyti radix extracts (250 mg/kg) were administrated, by gavage, for 28 days, and their effects on animal weight, liver and biliary functions, hepatic tissue and oxidative stress were investigated. Alter the acute toxicity testing, the dry extracts were placed in the GHS Category V (LD50>5000 mg/kg, p.o.). For the subacute toxicity testing, no death or any signs of toxicity were observed. Also, no significant differences in biochemical parameters were observed between control and treated groups. The observed histopathological lesions were non-specific and were not consistent with the data reported in the literature for PAs exposure. In conclusion, the administration for 28 days, of the tested extracts, in a dose which correspond to a PAs concentration over the limits imposed in some countries, produced no hepatic and biliary toxic effects. Further studies, extended over a longer period of time, are needed in order to determine the safety of plant extracts containing PAs.
引用
收藏
页码:1017 / 1023
页数:7
相关论文
共 30 条
[1]  
Agency E.M., 2014, EMA pharmacovigilance system manual, V44, P1
[2]   MONOCROTALINE-INDUCED BUDD-CHIARI SYNDROME IN MONKEYS [J].
ALLEN, JR ;
CARSTENS, LA .
AMERICAN JOURNAL OF DIGESTIVE DISEASES, 1971, 16 (02) :111-+
[3]   Risk assessment on the use of herbal medicinal products containing pyrrolizidine alkaloids [J].
Allgaier, Clemens ;
Franz, Stephanie .
REGULATORY TOXICOLOGY AND PHARMACOLOGY, 2015, 73 (02) :494-500
[4]   Scientific Opinion on Pyrrolizidine alkaloids in food and feed [J].
不详 .
EFSA JOURNAL, 2011, 9 (11)
[5]  
[Anonymous], 2016, EMAHMPC3287822016, P1
[6]  
[Anonymous], 2010, EMEA CHMPSWP15011520, P1
[7]  
[Anonymous], 1988, ENV HLTH CRIT, V80
[8]  
[Anonymous], 2013, OVERVIEW PYRROLIZIDI
[9]  
[Anonymous], 2002, OECD SERIES TESTING, V33
[10]  
Bull L. B., 1955, Australian Veterinary Journal, V31, P33, DOI 10.1111/j.1751-0813.1955.tb05488.x