Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes

被引:137
作者
Kovalenko, OV
Plug, AW
Haaf, T
Gonda, DK
Ashley, T
Ward, DC
Radding, CM
Golub, EI
机构
[1] YALE UNIV,SCH MED,DEPT GENET,NEW HAVEN,CT 06510
[2] YALE UNIV,SCH MED,DEPT BIOCHEM & MOLEC BIOPHYS,NEW HAVEN,CT 06510
关键词
ubiquitination; homologous recombination; meiosis; spermatocytes;
D O I
10.1073/pnas.93.7.2958
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Hsubc9, a human gene encoding a ubiquitin-conjugating enzyme, has been cloned. The 18-kDa HsUbc9 protein is homologous to the ubiquitin-conjugating enzymes Hus5 of Schizosaccharomyces pombe and Ubc9 of Saccharomyces cerevisiae, The Hsubc9 gene complements a ubc9 mutation of S. cerevisiae. It has been mapped to chromosome 16p13.3 and is expressed in many human tissues, with the highest levels in testis and thymus, According to the Ga14 two-hybrid system analysis, HsUbc9 protein interacts with human recombination protein Rad51, A mouse homolog, Mmubc9, encodes an amino acid sequence that is identical to the human protein, In mouse spermatocytes, MmUbc9 protein, like Rad51 protein, localizes in synaptonemal complexes, which suggests that Ubc9 protein plays a regulatory role in meiosis.
引用
收藏
页码:2958 / 2963
页数:6
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