Effective intranasal therapeutics and prophylactics with monoclonal antibody against lethal infection of H7N7 influenza virus

被引:20
作者
He, Fang [1 ]
Kumar, Subaschandrabose Rajesh [1 ]
Khader, Syed Musthaq Syed [1 ]
Tan, Yunrui [1 ]
Prabakaran, Mookkan [1 ]
Kwang, Jimmy [1 ,2 ]
机构
[1] Temasek Life Sci Lab, Singapore, Singapore
[2] Natl Univ Singapore, Dept Microbiol, Fac Med, Singapore 117604, Singapore
关键词
Monoclonal antibody; Intranasal therapeutics; H7; AIV; AVIAN INFLUENZA; H5N1; INFLUENZA; UNIVERSAL DETECTION; COMMERCIAL POULTRY; HUMAN-BEINGS; A VIRUS; IGA; ELISA; MICE; CONJUNCTIVITIS;
D O I
10.1016/j.antiviral.2013.08.003
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Recurrence of highly pathogenic avian influenza (HPAI) virus subtype H7 in humans and poultry continues to be a serious concern to public health. No effective prevention and treatment are currently available against H7 infection. One H7 monoclonal antibody, Mab 62 was selected and characterized. Mab 62 presented efficient neutralization activity against all six representative H7 strains tested, including the H7N9 strain from the recent outbreak in China. The epitope of 62 identified on H7 HA1 exists in all the human H7 strains, including the recent H7N9 strains from China. Mab 62 when administered passively, pre or post challenge with 5 MLD50 (50% mouse lethal dose) HPAI H7N7 influenza viruses could protect 100% of the mice from death. The efficacy of intranasal administration of the Mab was evaluated versus the intraperitoneal route. In the therapeutic study, body weight loss and virus load were reduced in intranasally inoculated mice, as compared to the intraperitoneal group. Intranasal administration results in early clearance of the virus from the lungs and completely prevents lung pathology of H7N7. The study confirmed that intranasal administration of Mob 62 is either an effective prophylactic or therapeutic means against H7 lethal infection. The results of epitope analysis suggest the potential of Mab 62 to be used for the efficacious prevention and treatment against the recent human H7N9 strains. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:207 / 214
页数:8
相关论文
共 34 条
[1]   Pathogenesis of avian influenza (H7) virus infection in mice and ferrets: Enhanced virulence of Eurasian H7N7 viruses isolated from humans [J].
Belser, Jessica A. ;
Lu, Xuihua ;
Maines, Taronna R. ;
Smith, Catherine ;
Li, Yan ;
Donis, Ruben O. ;
Katz, Jacqueline M. ;
Tumpey, Terrence M. .
JOURNAL OF VIROLOGY, 2007, 81 (20) :11139-11147
[2]   Infection With Highly Pathogenic H7 Influenza Viruses Results in an Attenuated Proinflammatory Cytokine and Chemokine Response Early After Infection [J].
Belser, Jessica A. ;
Zeng, Hui ;
Katz, Jacqueline M. ;
Tumpey, Terrence M. .
JOURNAL OF INFECTIOUS DISEASES, 2011, 203 (01) :40-48
[3]   Past, Present, and Possible Future Human Infection with Influenza Virus A Subtype H7 [J].
Belser, Jessica A. ;
Bridges, Carolyn B. ;
Katz, Jacqueline M. ;
Tumpey, Terrence M. .
EMERGING INFECTIOUS DISEASES, 2009, 15 (06) :859-865
[4]   INCREASED VIRULENCE OF A MOUSE-ADAPTED VARIANT OF INFLUENZA A/FM/1/47 VIRUS IS CONTROLLED BY MUTATIONS IN GENOME SEGMENTS 4, 5, 7, AND 8 [J].
BROWN, EG .
JOURNAL OF VIROLOGY, 1990, 64 (09) :4523-4533
[5]   Passive antibody therapy for infectious diseases [J].
Casadevall, A ;
Dadachova, E ;
Pirofski, L .
NATURE REVIEWS MICROBIOLOGY, 2004, 2 (09) :695-703
[6]   Avian influenza A virus (H7N7) associated with human conjunctivitis and a fatal case of acute respiratory distress syndrome. [J].
Fouchier, RAM ;
Schneeberger, PM ;
Rozendaal, FW ;
Broekman, JM ;
Kemink, SAG ;
Munstert, V ;
Kuiken, T ;
Rimmelzwaan, GF ;
Schutten, M ;
van Doornum, GJJ ;
Koch, G ;
Bosman, A ;
Koopmans, M ;
Osterhaus, ADME .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (05) :1356-1361
[7]   Receptor-Binding Profiles of H7 Subtype Influenza Viruses in Different Host Species [J].
Gambaryan, Alexandra S. ;
Matrosovich, Tatyana Y. ;
Philipp, Jennifer ;
Munster, Vincent J. ;
Fouchier, Ron A. M. ;
Cattoli, Giovanni ;
Capua, Ilaria ;
Krauss, Scott L. ;
Webster, Robert G. ;
Banks, Jill ;
Bovin, Nicolai V. ;
Klenk, Hans-Dieter ;
Matrosovich, Mikhail N. .
JOURNAL OF VIROLOGY, 2012, 86 (08) :4370-4379
[8]  
He F., 2013, INFLUENZA RES TREAT, V2013, P1
[9]   Immunohistochemical detection of Influenza virus infection in formalin-fixed tissues with anti-H5 monoclonal antibody recognizing FFWTILKP [J].
He, Fang ;
Du, Qingyun ;
Ho, YuenFern ;
Kwang, Jimmy .
JOURNAL OF VIROLOGICAL METHODS, 2009, 155 (01) :25-33
[10]   Complementary monoclonal antibody-based dot ELISA for universal detection of H5 avian influenza virus [J].
He, Fang ;
Soejoedono, Retno D. ;
Murtini, Sri ;
Goutama, Michael ;
Kwang, Jimmy .
BMC MICROBIOLOGY, 2010, 10