Molecular Detection of Human H7N9 Influenza A Virus Causing Outbreaks in China

被引：25

作者：

Wong, Chloe K. S. ^{[1
,2
]}

Zhu, Huachen ^{[1
,2
,3
,4
]}

Li, Olive T. W. ^{[1
,2
]}

Leung, Yin Hung C. ^{[1
,2
]}

Chan, Michael C. W. ^{[1
,2
,3
]}

Guan, Yi ^{[1
,2
,3
,4
]}

Peiris, Joseph S. M. ^{[1
,2
,3
]}

Poon, Leo L. M. ^{[1
,2
,3
]}

机构：

[1] Univ Hong Kong, LKS Fac Med, Influenza Res Ctr, Pokfulam, Hong Kong, Peoples R China

[2] Univ Hong Kong, LKS Fac Med, Sch Publ Hlth, Pokfulam, Hong Kong, Peoples R China

[3] Univ Hong Kong, Li Ka Shing Fac Med, State Key Lab Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China

[4] Shantou Univ, Coll Med, Int Inst Infect & Immun, Shantou, Guangdong, Peoples R China

来源：

CLINICAL CHEMISTRY | 2013年 / 59卷 / 07期

关键词：

HUMAN INFECTION; ORIGIN;

D O I：

10.1373/clinchem.2013.208975

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

BACKGROUND: A novel subtype of influenza A virus (H7N9) was recently identified in humans. The virus is a reassortant of avian viruses, but these human isolates contain mutations [hemagglutinin (HA) Q226L and PB2 E627K] that might make it easier for the virus to adapt to mammalian hosts. Molecular tests for rapid detection of this virus are urgently needed. METHODS: We developed a 1-step quantitative realtime reverse-transcription PCR assay to detect the novel human H7N9 virus. The primer set was specific to the hemagglutinin (HA) gene of the H7N9 viruses currently causing the outbreak in China and had mismatches to all previously known avian or mammalian H7 HA sequences. In addition, the assay was evaluated using influenza A viruses of various genetic backgrounds and other negative controls. RESULTS: The detection limit of the assay was approximately 0.04 TCID50 (median tissue culture infective dose) per reaction. The assay specificity was high and all negative control samples, including 8H7 viruses not closely related to the human H7N9 virus, tested negative. CONCLUSIONS: The established assay allows rapid detection of the novel human H7N9 virus, thereby allowing better pandemic preparedness. (C) 2013 American Association for Clinical Chemistry

引用

页码：1062 / 1067

页数：6

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