Fecal Microbiota Composition Differs Between Children With β-Cell Autoimmunity and Those Without

被引:439
作者
de Goffau, Marcus C. [1 ,2 ]
Luopajarvi, Kristiina [3 ]
Knip, Mikael [4 ,5 ,6 ,7 ]
Ilonen, Jorma [8 ,9 ]
Ruohtula, Terhi [3 ]
Harkonen, Taina [4 ,5 ]
Orivuori, Laura [3 ]
Hakala, Saara [3 ]
Welling, Gjalt W. [1 ,2 ]
Harmsen, Hermie J. [1 ,2 ]
Vaarala, Outi [3 ]
机构
[1] Univ Groningen, Univ Med Ctr Groningen, Dept Med Microbiol, NL-9713 AV Groningen, Netherlands
[2] Univ Groningen, Groningen, Netherlands
[3] Natl Inst Hlth & Welf, Dept Vaccinat & Immune Protect, Immune Response Unit, Helsinki, Finland
[4] Univ Helsinki, Childrens Hosp, Helsinki, Finland
[5] Univ Helsinki, Cent Hosp, Helsinki, Finland
[6] Folkhalsan Res Ctr, Helsinki, Finland
[7] Tampere Univ Hosp, Dept Pediat, Tampere, Finland
[8] Univ Turku, Immunogenet Lab, Turku, Finland
[9] Univ Eastern Finland, Dept Clin Immunol, Kuopio, Finland
关键词
BACTERIAL TRANSLOCATION; INTESTINAL MICROBIOTA; DIABETES-MELLITUS; SEQUENCE DATA; GUT; BUTYRATE; RISK; BIFIDOBACTERIA; FERMENTATION; MECHANISMS;
D O I
10.2337/db12-0526
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The role of the intestinal microbiota as a regulator of autoimmune diabetes in animal models is well-established, but data on human type 1 diabetes are tentative and based on studies including only a few study subjects. To exclude secondary effects of diabetes and FILA risk genotype on gut microbiota, we compared the intestinal microbiota composition in children with at least two diabetes-associated autoantibodies (n = 18) with autoantibody-negative children matched for age, sex, early feeding history, and HLA risk genotype using pyrosequencing. Principal component analysis indicated that a low abundance of lactate-producing and butyrate-producing species was associated with beta-cell autoimmunity. In addition, a dearth of the two most dominant Bifidobacterium species, Bifidobacterium adolescentis and Bifidobacterium pseudocatenulatum, and an increased abundance of the Bacteroides genus were observed in the children with beta-cell autoimmunity. We did not find increased fecal calprotectin or IgA as marker of inflammation in children with beta-cell autoimmunity. Functional studies related to the observed alterations in the gut microbiome are warranted because the low abundance of bifidobacteria and butyrate-producing species could adversely affect the intestinal epithelial barrier function and inflammation, whereas the apparent importance of the Bacteroides genus in development of type 1 diabetes is insufficiently understood. Diabetes 62:1238-1244, 2013
引用
收藏
页码:1238 / 1244
页数:7
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共 47 条
  • [41] Lactobacillus johnsonii N6.2 Mitigates the Development of Type 1 Diabetes in BB-DP Rats
    Valladares, Ricardo
    Sankar, Dhyana
    Li, Nan
    Williams, Emily
    Lai, Kin-Kwan
    Abdelgeliel, Asmaa Sayed
    Gonzalez, Claudio F.
    Wasserfall, Clive H.
    Larkin, Joseph, III
    Schatz, Desmond
    Atkinson, Mark A.
    Triplett, Eric W.
    Neu, Josef
    Lorca, Graciela L.
    [J]. PLOS ONE, 2010, 5 (05):
  • [42] Microarray Analysis and Barcoded Pyrosequencing Provide Consistent Microbial Profiles Depending on the Source of Human Intestinal Samples
    van den Bogert, Bartholomeus
    de Vos, Willem M.
    Zoetendal, Erwin G.
    Kleerebezem, Michiel
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (06) : 2071 - 2080
  • [43] Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy
    Wang, Qiong
    Garrity, George M.
    Tiedje, James M.
    Cole, James R.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (16) : 5261 - 5267
  • [44] Risk factors of development of gut-derived bacterial translocation in thermally injured rats
    Wang, Zhong-Tang
    Yao, Yong-Ming
    Xiao, Guang-Xia
    Sheng, Zhi-Yong
    [J]. WORLD JOURNAL OF GASTROENTEROLOGY, 2004, 10 (11) : 1619 - 1624
  • [45] Innate immunity and intestinal microbiota in the development of Type 1 diabetes
    Wen, Li
    Ley, Ruth E.
    Volchkov, Pavel Yu.
    Stranges, Peter B.
    Avanesyan, Lia
    Stonebraker, Austin C.
    Hu, Changyun
    Wong, F. Susan
    Szot, Gregory L.
    Bluestone, Jeffrey A.
    Gordon, Jeffrey I.
    Chervonsky, Alexander V.
    [J]. NATURE, 2008, 455 (7216) : 1109 - U10
  • [46] Sampling and pyrosequencing methods for characterizing bacterial communities in the human gut using 16S sequence tags
    Wu, Gary D.
    Lewis, James D.
    Hoffmann, Christian
    Chen, Ying-Yu
    Knight, Rob
    Bittinger, Kyle
    Hwang, Jennifer
    Chen, Jun
    Berkowsky, Ronald
    Nessel, Lisa
    Li, Hongzhe
    Bushman, Frederic D.
    [J]. BMC MICROBIOLOGY, 2010, 10
  • [47] Improved extraction of PCR-quality community DNA from digesta and fecal samples
    Yu, ZT
    Morrison, M
    [J]. BIOTECHNIQUES, 2004, 36 (05) : 808 - +