Unexpectedly high bacterial diversity in decaying wood of a conifer as revealed by a molecular method

被引:44
作者
Zhang, Han-Bo [1 ,2 ,3 ]
Yang, Ming-Xia [1 ]
Tu, Ran [1 ]
机构
[1] Yunnan Univ, Dept Biol, Kunming 650091, Yunnan Province, Peoples R China
[2] Yunnan Univ, Lab Conservat & Utilizat Bioresources, Kunming 650091, Peoples R China
[3] Yunnan Univ, Minist Educ, Key Lab Microbial Resources, Kunming 650091, Peoples R China
关键词
Heartwood; Sapwood; Keteleeria evelyniana; Bacterial community; 16S rRNA gene;
D O I
10.1016/j.ibiod.2008.06.001
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bacterial communities in decaying sapwood and heartwood of Keteleeria evelyniana were demonstrated through constructing rRNA gene libraries. At 3% of sequence difference, 81 operational taxonomic units (OTUs) were identified from 91 partial 16S rRNA gene fragments. These clones belonged to Proteobacteria (63.7%), Actinobacteria (13.2%), Acidobacteria (8.8%), Firmicutes (3.3%), Chlamydiae (5.5%), Chloiroflexi (3.3%), Verrucomicrobia (1.1%) and Cyanobacteria (1.1%). The heartwood displayed higher species richness than the sapwood. No OTU overlapped between these two parts. Most alpha- and beta-, and all delta-proteobacterial clones occurred in the heartwood, whereas gamma-proteobacteria mainly occurred in the sapwood. Chlamydiae and Verrucomicrobia exclusively appeared in the heartwood, but Chloroflexi, Cyanobacteria, and Firmicutes, distributed in the sapwood. Clones belonging to Aciclobacteria and Actinobacteria were also divided into distinct subgroups between in the sapwood and in the heartwood. Based on the present species definition, we estimated the wood-inhibiting bacteria of K evelyniana achieved up 1056 species (ranging from 452 to 2647). Only small number of sequences (13/91) can be found the nearest neighbor previously cultured and named: thereby, the majority of wood-inhabiting bacteria still remains to be uncovered. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:471 / 474
页数:4
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