A comparative study of proliferation and osteogenic differentiation of rat adipose-derived stem cells in β-tricalcium phosphate (β-TCP), forsterite (Mg2SiO4) and clinoenstatite (MgSiO3)

被引：8
作者：
Leng Bin [1 ,2 ,3 ]
Jin XiaoGang [4 ]
Lin QiuXia [2 ,3 ]
Chen Lei [4 ]
Wang Yan [2 ,3 ]
Du ZhiYan [2 ,3 ]
Lin KaiLi [4 ]
Chang Jiang [4 ]
Gu XiaoMing [1 ]
Wang ChangYong [2 ,3 ]
机构：
[1] Peoples Armed Police Gen Hosp, Dept Implantol, Beijing 100039, Peoples R China
[2] Acad Mil Med Sci, Dept Adv Interdisciplinary Studies, Inst Basic Med Sci, Beijing 100850, Peoples R China
[3] Acad Mil Med Sci, Tissue Engn Res Ctr, Beijing 100850, Peoples R China
[4] Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2013年 / 58卷 / 24期
基金：
中国国家自然科学基金;
关键词：
forsterite; clinoenstatite; adipose-derived stem cells; osteogenesis; bone; bone tissue engineering; IN-VITRO; OSTEOBLAST DIFFERENTIATION; MECHANICAL-PROPERTIES; SIGNALING PATHWAYS; STROMAL CELLS; BONE; AKERMANITE; TISSUE; ACTIVATION; TITANIUM;
D O I：
10.1007/s11434-013-5874-3
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In this study, the effects of forsterite and clinoenstatite powder extracts on the proliferation and osteogenic differentiation of rat adipose-derived stem cells (ASCs) were investigated and compared with the beta-tricalcium phosphate (beta-TCP) powder extracts. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and live-dead staining were performed to evaluate the viability and proliferation of rat ASCs. Osteogenic differentiation of rat ASCs were assayed by alkaline phosphatase (ALP) staining and ALP activity test. The expression of osteogenic marker genes (alkaline phosphatase (ALP), runt related transcription factor 2 (Runx2), collagen type I alpha 1 (Col1 alpha 1), secreted phosphoprotein1 (Spp1, osteopontin), integrin binding sialoprotein (Ibsp), bone gla protein (Bglap)) were detected by real-time polymerase chain reaction (PCR). The MTT assay and the live-dead staining showed that all the three ceramics possessed good cytocompatibility with rat ASCs. Furthermore, forsterite and clinoenstatite promoted the proliferation of rat ASCs compared with beta-TCP. The results of the ALP activity test and the real-time PCR demonstrated that forsterite and clinoenstatite promoted the osteogenic differentiation of rat ASCs. These results suggested that forsterite and clinoenstatite are bioactive ceramics that may be used for preparation of bone tissue engineering (BTE) scaffolds.
引用
收藏
页码：3033 / 3042
页数：10
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