Searching biomarker candidates in serum using multidimensional native chromatography. II Method evaluation with Alport syndrome and severe inflammation

被引：15

作者：

Baum, Adrienne ^{[1
]}

Pohl, Michael ^{[2
]}

Kreusch, Stefan ^{[1
]}

Cumme, Gerhard A. ^{[1
]}

Ditze, Guenter

Misselwitz, Joachim ^{[2
]}

Kiehntopf, Michael ^{[3
]}

Udby, Lene ^{[4
]}

Meier-Hellmann, Andreas ^{[5
]}

Rhode, Heidrun ^{[1
]}

机构：

[1] Univ Jena, Fac Med, Inst Biochem, D-07740 Jena, Germany

[2] Univ Jena, Fac Med, Childrens Hosp, D-07740 Jena, Germany

[3] Univ Jena, Fac Med, Inst Clin Chem & Lab Med, D-07740 Jena, Germany

[4] Rigshosp, Copenhagen Univ Hosp, Dept Hematol, Granulocyte Res Lab, Copenhagen, Denmark

[5] Helios Clin GmbH, Erfurt, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 876卷 / 01期

关键词：

Biomarker search; Multidimensional chromatography; Serum; Alport syndrome; Sepsis; Albumin;

D O I：

10.1016/j.jchromb.2008.10.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Biomarker search using multidimensional native liquid fractionation of serum in microplates was evaluated. From different donors, homologous sample fractions with UV absorbance depending on state of illness were selected, and their constituents were identified and quantitated by MS. Analysis of sera of patients with Alport syndrome and severe inflammation proved the reliability of the method by confirming characteristic alterations. Moreover, 23 new marker candidates were detected for Alport syndrome, some of them being involved in matrix degradation and repair, and 33 new candidates for severe inflammation, among them alpha 1B-glycoprotein cysteine-rich secretory protein and an apparently low molecular-weight albumin variant. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：31 / 40

页数：10

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