Self-assembled nanoparticles based on amphiphilic chitosan derivative and hyaluronic acid for gene delivery

被引：39
作者：
Liu, Ya [1 ]
Kong, Ming [1 ]
Cheng, Xiao Jie [1 ]
Wang, Qian Qian [1 ]
Jiang, Li Ming [1 ]
Chen, Xi Guang [1 ]
机构：
[1] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2013年 / 94卷 / 01期
基金：
中国国家自然科学基金;
关键词：
OCMCS; HA; DNA; NPs; Gene vector; IN-VITRO EVALUATION; TRANSFECTION EFFICIENCY; DNA NANOPARTICLES; THERAPY; POLYMERS; CARRIERS; VECTORS; CD44;
D O I：
10.1016/j.carbpol.2012.12.058
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The present work described nanoparticles (NPs) made of oleoyl-carboxymethy-chitosan (OCMCS)/hyaluronic acid (HA) using coacervation process as novel potential carriers for gene delivery. An N/P ratio of 5 and OCMCS/HA weight ratio of 4 were the optimal conditions leading to the smallest (164.94 nm), positive charged (+14.2 mV) and monodispersed NPs. OCMCS-HA/DNA (OHD) NPs showed higher in vitro DNA release rates and increased cellular uptake by Caco-2 cells due to the HA involved in NPs. The MIT survival assay indicated no significant cytotoxicity. The transfection efficiency of OHD NPs was 5-fold higher than OCMCS/DNA (OD) NPs; however, it decreased significantly in the presence of excess free HA. The results indicated that OHD NPs internalized in Caco-2 cells were mediated by the hyaluronan receptor CD44. The data obtained in the present research gave evidence of the potential of OHD NPs for the targeting and further transfer of genes to the epithelial cells. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：309 / 316
页数：8
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